氯化钴对原代大鼠肺动脉成纤维细胞的影响  

Effect of Cobalt Chloride on Pulmonary Arterial Fibroblasts of Rat

作　　者：柴晓宇[1] 许慧莹[1] 刘钟桧 易亮[1] 刘新民[1] 

机构地区：[1]北京大学第一医院老年内科,北京100034

出　　处：《现代生物医学进展》2017年第15期2801-2804,共4页Progress in Modern Biomedicine

基　　金：国家自然科学基金项目(81270114)

摘　　要：目的:探究氯化钴对于原代大鼠肺动脉成纤维细胞(PAF)的增殖、迁移、表型转化作用的影响。方法:分离培养大鼠肺动脉成纤维细胞,利用氯化钴刺激PAF细胞,并通过MTT、细胞划痕、Transwell、表型转化标志蛋白测定以及PI3K/Akt信号通路蛋白的变化研究氯化钴对PAF的影响。结果:MTT结果显示,与对照组相比,氯化钴可以抑制大鼠肺动脉成纤维细胞的增殖活性(P<0.001),并呈浓度依赖性。划痕实验及Transwell实验提示较高氯化钴(200μmol·L^(-1))处理PAF细胞后可以抑制细胞迁移。随浓度增加,氯化钴可以抑制PAF的P110α、p-Akt蛋白表达。结论:氯化钴对于体外培养的原代大鼠肺动脉成纤维细胞的增殖、迁移和表型转化特性有一定的抑制作用,这可能与氯化钴抑制PI3K/Akt信号通路的表达有关。Objective：Study the effects of cobalt chloride on the proliferation,migration and phenotype transformation on the primary pulmonary artery fibroblasts （PAF） from rat.Methods：Using cobalt chloride to stimulate the established PAF.Value the effects of cobalt chloride on PAF by MTT,cell scratch,Transwell,phenotype marker protein expression and PI3K/Akt signaling pathway proteins expressions.Results：Compared with the control group,MTT results showed that cobalt chloride could decrease the proliferation activity of PAF in a concentration-dependent manner.Scratch and Transwell assays showed that higher concentrations of cobalt chloride could inhibit the migration of PAF.Cobalt chloride could decrease the protein expressions of P110α and p-Akt in a concentration-dependent manner.Conclusions：Cobalt chloride could inhibit the proliferation,migration and phenotype conversion characteristics of PAF by affecting the expression of PI3K/Akt signaling pathway which might involve in the regulation of cell behaviors.

关 键 词：氯化钴 肺动脉成纤维细胞 增殖 P13K 

分 类 号：Q95-33[生物学—动物学] Q47