异氟烷对小鼠星型胶质细胞Sirt1和MAO-A基因表达的影响  

Effect of Isoflurane on the Expression of Sirt1/MAO-A in Cultured Astrocytes

作　　者：何宏[1] 顾婷[2] 薛姗姗[1] 王化宁[1] 彭正午[1] 周翠红[1] 

机构地区：[1]第四军医大学西京医院心身科,陕西西安710032 [2]第四军医大学西京医院麻醉科,陕西西安710032

出　　处：《现代生物医学进展》2017年第15期2817-2820,共4页Progress in Modern Biomedicine

基　　金：国家自然科学基金项目(81401109;81571309)

摘　　要：目的:探讨异氟烷对小鼠星形胶质细胞Sirt1和MAO-A基因表达的影响。方法:给予体外培养的新生小鼠原代星形胶质细胞不同浓度异氟烷处理,实验分为对照组和异氟烷处理组(0.5ISO、1.0ISO、1.5ISO),其中异氟烷组细胞分别给予0.5 MAC、1.0MAC和1.5 MAC三个浓度的异氟烷处理2小时,对照组给予O_2处理2小时,然后提取细胞RNA和蛋白检测Sirt1和MAO-A的mRNA和蛋白表达变化。结果:与对照组相比,异氟烷下调小鼠星形胶质细胞的Sirt1和MAO-A mRNA和蛋白水平,异氟烷浓度越大下调越明显。结论:异氟烷可抑制小鼠星形胶质细胞的Sirt1和MAO-A基因表达。Objective：To explore the effect of isoflurane on the expression of Sirtl/MAO-A of Astrocytes.Methods：Cultured astrocytes from newborn mice were divided into control and isoflurane groups：control group was treated with O2 for 2 hours and isoflurane treatment groups （0.5ISO,1.0ISO,1.5ISO） were treated with 0.5 MAC,1.0 MAC and 1.5 MAC of isoflurane for 2 hours respectively.Then total RNA and protein was extracted from cells and the mRNA and protein levels of Sirtl and MAO-A were measured by Real-time PCR and Western blot respectively.Results：（1） Compared with the control,Isoflurane significantly down-regulated the mRNA as well as protein levels of Sirtl and MAO-A in the cultured astrocytes;（2） The effect of isoflurane revealed a dose effect---compared with 0.5ISO group,cells treated with 1.0MAC and 1.5 MAC of isoflurane suffered more influence.Conclusion：Isoflurane exposure suppressed the expression of Sirt1/MAO-A of astrocytes from newborn mice brain,which is important for the isoflurane narcotherapy in depressive.

关 键 词：异氟烷 星形胶质细胞 SIRT1 MAO—A 

分 类 号：R33[医药卫生—人体生理学] R74[医药卫生—基础医学]