Chemical Constituents from Mycelia and Spores of Fungus Cordyceps cicadae  

作　　者：Jia-hui Wang Zhong-liang Zhang Yu-qin Wang Min Yang Chun-hua Wang Xu-wen Li Yue-wei Guo 

机构地区：[1]School of Pharmacy,Binzhou Medical University,Yantai 264003,China [2]State Key Laboratory of Drug Research,Shanghai Institute of Materia Medica,Chinese Academy of Seiences,Shanghai 201203,China [3]Zhejiang Bioasia Life Science Research Institute,Pinghu 314200,China [4]Zhejiang Bioasia Pharmaceutical Co.,Ltd.,Pinghu 314200,China

出　　处：《Chinese Herbal Medicines》2017年第2期188-192,共5页中草药（英文版）

基　　金：National Natural Science Foundation of China(No.81520108028,81273430,41306130,81302692,41676073,81603022);SCTSM Project(No.14431901100,15431901000);Institutes for Drug Discovery and Development,Chinese Academy of Sciences(No.CASIMM0120152039);SKLDR/SIMM Projects(SIMM 1501ZZ-03);“Youth Innovation Promotion Association”from Chinese Academy of Sciences(No.2016258);“Young Talent Supporting Project”from China Association for Science and Technology(No.2016QNRC001);Shanghai“Pujiang Program”(No.16PJ1410600)

摘　　要：Objective To isolate and identify the chemical constituents from mycelia and spores of the fungus Cordyceps cicadae,respectively.Methods The chemical constituents were isolated and purified by repeated silica gel,Sephadex LH-20,and reversed phase HPLC.The structures of these compounds were elucidated on the basis of extensive spectroscopic analysis,including 1 D and 2D NMR.Results Nine known sterols such as ergosterol（1）,ergosterol peroxide（2）,9,11-dehydroergosterol peroxide（3）,3β,5α,9α-trihydroxy-（22E,24R）-ergosta-7,22-dien-6-one（4）,3β,5α,9α,14α-tetrahydroxy-（22E,24R）-ergosta-7,22-dien-6-one（5）,5 α,6α-e poxy-（22 E,,24 R）-e rgosta-8（14）,22-diene-3α,7α-diol （6）, 3β,5α,6β-（22E,24R）-ergosta-7,22-dien-3,5,6-triol （7）, 3β,5α,6α-6- methoxyergosta-（22E,24R）-7,22-diene-3,5-diol （8）, 4-hydroxy-17R-methylincisterol （9）, together with a resorcinol derivative, 5-n-nonadecylresorcinol （10）, a cydo- desipeptide, beauvericin （11）, and a nucleoside, N^6-（2-hydroxyethyl）adenosine （12） were successively isolated from the cultivated C. cicadaemycelia and spores. Conclusion Compounds 3-10 are reported for the first time from the title sample, beauvericin exhibits significant cytotoxicity against human leukemia cell line and human lung cancer cell line.Objective To isolate and identify the chemical constituents from mycelia and spores of the fungus Cordyceps cicadae,respectively.Methods The chemical constituents were isolated and purified by repeated silica gel,Sephadex LH-20,and reversed phase HPLC.The structures of these compounds were elucidated on the basis of extensive spectroscopic analysis,including 1 D and 2D NMR.Results Nine known sterols such as ergosterol（1）,ergosterol peroxide（2）,9,11-dehydroergosterol peroxide（3）,3β,5α,9α-trihydroxy-（22E,24R）-ergosta-7,22-dien-6-one（4）,3β,5α,9α,14α-tetrahydroxy-（22E,24R）-ergosta-7,22-dien-6-one（5）,5 α,6α-e poxy-（22 E,,24 R）-e rgosta-8（14）,22-diene-3α,7α-diol （6）, 3β,5α,6β-（22E,24R）-ergosta-7,22-dien-3,5,6-triol （7）, 3β,5α,6α-6- methoxyergosta-（22E,24R）-7,22-diene-3,5-diol （8）, 4-hydroxy-17R-methylincisterol （9）, together with a resorcinol derivative, 5-n-nonadecylresorcinol （10）, a cydo- desipeptide, beauvericin （11）, and a nucleoside, N^6-（2-hydroxyethyl）adenosine （12） were successively isolated from the cultivated C. cicadaemycelia and spores. Conclusion Compounds 3-10 are reported for the first time from the title sample, beauvericin exhibits significant cytotoxicity against human leukemia cell line and human lung cancer cell line.

关 键 词：beauvericin Cordyceps cicadae cyclodesipeptide cytotoxicity sterols 

分 类 号：R284[医药卫生—中药学]