巨噬细胞通过直接物理粘附和机械牵引来介导脑血管破裂的修复  

Macrophages Mediate the Repair of Brain Vascular Rupture through Direct Physical Adhesion and Mechanical Traction

作　　者：Chi Liu Chuan Wu Qifen Yang Jing Gao Li Li 杨德琴[2] 罗凌飞 

机构地区：[1]不详 [2]重庆医科大学 [3]西南大学

出　　处：《科学新闻》2017年第4期48-48,共1页Science News

基　　金：国家重点基础研究发展规划项目(2015CB942800);国家自然科学基金重大研究计划(项目号:91539201,31330051,31130038,31371473,31571508);科技部等项目的资助

摘　　要：出血性脑卒中和脑出血是由脑血管破裂引起的。快速修复这种破裂是最有希望的治疗方法。由于缺乏高分辨率的体内实时研究，脑血管修复过程中的动态细胞事件仍然未知。Hemorrhagic stroke and brain microbleeds are caused by cerebrovascular ruptures. Fast repair of such ruptures is the most promising therapeutic approach. Due to a lack of high-resolution in vivo real-time studies, the dynamic cellular events involved in cerebrovascular repair remain unknown. Here, we have developed a cerebrovascular rupture system in zebrafish by using multi-photon laser, which generates a lesion with two endothelial ends. In vivo time-lapse imaging showed that a macrophage arrived at the lesion and extended filopodia or lamellipodia to physically adhere to both endothelial ends. This macrophage generated mechanical traction forces to pull the endothelial ends and facilitate their ligation, thus mediating the repair of the rupture. Both depolymerization of microfilaments and inhibition of phosphatidylinositide 3-kinase or Rac1 activity disrupted macrophage-endothelial adhesion and impaired cerebrovascular repair. Our study reveals a hitherto unexpected role for macrophages in mediating repair of cerebrovascular ruptures through direct physical adhesion and mechanical traction.

关 键 词：巨噬细胞 修复 破裂 牵引 机械 物理 高分辨率 脑出血 

分 类 号：Q26[生物学—细胞生物学]