小分子化合物诱导人胃上皮细胞直接转化为内胚层多潜能祖细胞  

Conversion of Human Gastric Epithelial Cells to Multipotent Endodermal Progenitors using Defined Small Molecules

作　　者：王韫芳[1,2] Jinhua Qin Shuyong Wang Wencheng Zhang Jialei Duan Jing Zhang Xin Wang Fang Yan Mingyang Chang Xiaofang Liu Bo Feng Jiang Liu 裴雪涛[1,4,5] 

机构地区：[1]军事医学科学院野战输血研究所 [2]军事医学科学院野战输血研究所组织工程研究室 [3]不详 [4]军事医学科学院全军干细胞与再生医学重点实验室 [5]国家863计划"干细胞与组织工程重大项目"总体专家组

出　　处：《科学新闻》2017年第4期67-67,共1页Science News

基　　金：中国科学技术部;国家自然科学基金委;中国科学院;广东省的基金支持

摘　　要：内胚层干／祖细胞是肝脏、胰腺、小肠等内脏器官的共同起源，在再生医学的基础研究和临床应用中具有广阔的应用前景。因此，寻找一种安全高效的获取内胚层干/祖细胞的方式将具有极其重要的意义。Endodermal stem/progenitor cells have diverse potential applications in research and regenerative medicine, so a readily available source could have widespread uses. Here we describe derivation of human induced endodermal progenitor cells (hiEndoPCs) from gastrointestinal epithelial cells using a cocktail of defined small molecules along with support from tissue-specific mesenchymal feeders. The hiEndoPCs show clonal expansion in culture and give rise to hepatocytes, pancreatic endocrine cells, and intestinal epithelial cells when treated with defined soluble molecules directing differentiation. The hiEndoPC-derived hepatocytes are able to rescue liver failure in Fah(-/-) Rag2(-/-) mice after transplantation, and, unlike hESCs, transplanted hiEndoPCs do not give rise to teratomas. Since human gastric epithelial cells are readily available from donors of many ages, this conversion strategy can generate clonally expandable cell populations with a variety of potential applications, including personalized drug screening and therapeutic strategies for liver failure and diabetes.

关 键 词：上皮细胞 直接转化 化合物 诱导 分子 临床应用 内脏器官 基础研究 

分 类 号：O623.11[理学—有机化学]