独特加工方式产生长非编码RNASPAs选择性结合多个剪接调控因子  

Unusual Processing Generates SPA LncRNAs that Sequester Multiple RNA Binding Proteins

作　　者：Huang Wu Qing-Fei Yin Zheng Luo Run-Wen Yao Chuan-Chuan Zheng Jun Zhang Jian-Feng Xiang Li Yang 陈玲玲[2] 

机构地区：[1]不详 [2]中国科学院上海生命科学研究院生物化学与细胞生物学研究所

出　　处：《科学新闻》2017年第4期94-94,共1页Science News

基　　金：国家科技部;基金委的经费支持

摘　　要：人类基因组中存在大量被称为基因组“暗物质”的非编码序列，越来越多的非编码序列功能被科学家发掘。We identify a type of polycistronic transcript-derived long noncoding RNAs (lncRNAs) that are 50 small nucleolar RNA (snoRNA) capped and 30 polyadenylated (SPAs). SPA processing is associated with nascent mRNA 30 processing and kinetic competition between XRN2 trimming and Pol II elongation. Following cleavage/polyadenylation of its upstream gene, the downstream uncapped pre-SPA is trimmed by XRN2 until this exonuclease reaches the co-transcriptionally assembled snoRNP. This snoRNP complex prevents further degradation, generates a snoRNA 50 end, and allows continuous Pol II elongation. The imprinted 15q11-q13 encodes two SPAs that are deleted in Prader-Willi syndrome (PWS) patients. These lncRNAs form a nuclear accumulation that is enriched in RNA binding proteins (RBPs) including TDP43, RBFOX2, and hnRNP M. Generation of a human PWS cellular model by depleting these lncRNAs results in altered patterns of RBPs binding and alternative splicing. Together, these results expand the diversity of lncRNAs and provide additional insights into PWS pathogenesis.

关 键 词：编码 加工方式 选择性 人类基因组 因子 剪接 生长 暗物质 

分 类 号：Q987[生物学—遗传学]