新生环形RNA生成与RNA聚合酶Ⅱ转录的偶联机制  

The Biogenesis of Nascent Circular RNAs

作　　者：Yang Zhang Wei Xue Xiang Li Jun Zhang Siye Chen Jia-Lin Zhang 杨力[2] 陈玲玲[3] 

机构地区：[1]不详 [2]中国科学院-德国马普学会计算生物学伙伴研究所 [3]中国科学院上海生命科学研究院生物化学与细胞生物学研究所

出　　处：《科学新闻》2017年第4期142-142,共1页Science News

基　　金：国家自然科学基金委;科技部;中科院的经费支持

摘　　要：环形RNA是一类通过反向剪接所形成的闭合环状RNA分子。由于环形RNA特殊的分子结构，其生成和代谢需要深入研究。Steady-state circular RNAs (circRNAs) have been mapped to thousands of genomic loci in mammals. We studied circRNA processing using metabolic tagging of nascent RNAs with 4-thiouridine (4sU). Strikingly, the efficiency of circRNA processing from pre-mRNA is extremely low endogenously. Additional studies revealed that back-splicing outcomes correlate with fast RNA Polymerase II elongation rate and are tightly controlled by cis-elements in vivo. Additionally, prolonged 4sU labeling in cells shows that circRNAs are largely processed post-transcriptionally and that circRNAs are stable. Circular RNAs that are abundant at a steady-state level tend to accumulate. This is particularly true in cells, such as neurons, that have slow division rates. This study uncovers features of circRNA biogenesis by investigating the link between nascent circRNA processing and transcription.

关 键 词：RNA 环形 联机 转录 聚合 新生 分子结构 酶 

分 类 号：Q522[生物学—生物化学]