移植神经干细胞分化的神经元功能性整合到清醒猕猴听觉回路  

Neurons Differentiated from Transplanted Stem Cells Respond Functionally to Acoustic Stimuli in the Awake Monkey Brain

作　　者：Jing-kuan Wei Wen-chao Wang Rong-wei Zhai Yu-hua Zhang Shang-chuan Yang Joshua Rizak Ling Li Li-qi Xu Li Liu Ming-ke Pan Ying-zhou Hu Abdelaziz Ghanemi Jing Wu Li-chuan Yang Hao Li Long-bao Lv Jia-li Li Yong-gang Yao Lin Xu Xiao-li Feng Yong Yin Dong-dong Qin 胡新天[2] 王正波[2] 

机构地区：[1]不详 [2]中国科学院昆明动物研究所

出　　处：《科学新闻》2017年第4期150-150,共1页Science News

基　　金：科技部973计划;国家自然科学基金;国家自然科学基金委重点培育计划;中科院先导B等资助

摘　　要：长期以来，中枢神经系统的损伤后再生与修复一直是神经科学领域的难题与科学家们研究的焦点。神经干细胞的概念出现后，具有多向分化潜能的干细胞拥有分化成神经元、替代死亡的神经元，恢复受损神经网络功能的潜能，并因此成为神经退行性疾病和脑损伤替代治疗研究的热点。Here, we examine whether neurons differentiated from transplanted stem cells can integrate into the host neural network and function in awake animals, a goal of transplanted stem cell therapy in the brain. We have developed a technique in which a small 'hole'' is created in the inferior colliculus (IC) of rhesus monkeys, then stem cells are transplanted in situ to allow for investigation of their integration into the auditory neural network. We found that some transplanted cells differentiated into mature neurons and formed synaptic input/output connections with the host neurons. In addition, c-Fos expression increased significantly in the cells after acoustic stimulation, and multichannel recordings indicated IC specific tuning activities in response to auditory stimulation. These results suggest that the transplanted cells have the potential to functionally integrate into the host neural network.

关 键 词：中枢神经系统 细胞分化 元功能 听觉 猕猴 清醒 整合 移植 

分 类 号：Q426[生物学—神经生物学]