氧化还原感应蛋白WhiB6调控结核性肉芽肿的发生和形成过程  

Mycobacterial WhiB6 Differentially Regulates ESX-1 and the Dos Regulon to Modulate Granuloma Formation and Virulence in Zebrafish

作　　者：Zhenkang Chen Yangbo Hu Bridgette M.Cumming Pei Lu Lipeng Feng Jiaoyu Deng Adrie J.C.Steyn 陈士云[2,3] 

机构地区：[1]不详 [2]中国科学院武汉病毒研究所 [3]中国科学院武汉病毒研究所病原细菌学科组

出　　处：《科学新闻》2017年第4期159-159,共1页Science News

基　　金：中国科学院重点部署项目;国家自然科学基金项目的资助

摘　　要：结核分枝杆菌（Mycobacterium tuberculosis,Mtb）感染人后主要形成潜伏感染，机体的免疫系统在与Mtb的相互作用过程中会形成结核性肉芽肿，但结核性肉芽肿发生和形成过程的调控机制尚不清楚。During the course of infection, Mycobacterium tuberculosis (Mtb) is exposed to diverse redox stresses that trigger metabolic and physiological changes. How these stressors are sensed and relayed to the Mtb transcriptional apparatus remains unclear. Here, we provide evidence that WhiB6 differentially regulates the ESX-1 and DosR regulons through its Fe-S cluster. When challenged with NO, WhiB6 continually activates expression of the DosR regulons but regulates ESX-1 expression through initial activation followed by gradual inhibition. Comparative transcriptomic analysis of the holo-and reduced apo-WhiB6 complemented strains confirms these results and also reveals that WhiB6 controls aerobic and anaerobic metabolism, cell division, and virulence. Using the Mycobacterium marinum zebrafish infection model, we find that holo-and apo-WhiB6 modulate levels of mycobacterial infection, granuloma formation, and dissemination. These findings provide fresh insight into the role of WhiB6 in mycobacterial infection, dissemination, and disease development.

关 键 词：结核 氧化还原 肉 蛋白 感应 相互作用 免疫系统 调控机制 

分 类 号：Q510.3[生物学—生物化学]