水解酪素对藏系绵羊瘤胃液体外产气及纤维素分解率的试验研究  

Research on Cellulose-decomposing and Methanogenesis in Vitro within Rumen Fluid of Tibetan Sheep by Added Hydrolyzed Casein

作　　者：田发益[1] 巴平[1] 

机构地区：[1]西藏农牧学院生物技术中心,西藏林芝860000

出　　处：《中国兽医杂志》2017年第3期47-50,共4页Chinese Journal of Veterinary Medicine

基　　金：国家自然科学基金(31360557)

摘　　要：反刍动物瘤胃中生产甲烷和纤维素分解两类微生物对环境和家畜起着重要的作用。生产甲烷不仅消耗养分,同时也是温室气体的主要来源;纤维素分解菌提供反刍家畜主要的碳水化合物。提高纤维素分解菌的活力是本研究的重点之一,同时掌握对甲烷产气菌的影响程度。通过瘤胃胃管抽取藏系绵羊的瘤胃液,采用体外产气装置进行产气量测定;通过瘤胃液中放置无纺织纤维袋以测定纤维素消失率;采用厌氧培养进行瘤胃细菌菌落记数的方法进行测定分析。结果表明,添加一定量的水解酪素后,体外产气量增加22.35%,P=0.433,差异不显著;对总纤维素25.8%的消失率进行t检验,试验组和对照组组间P=0.043,差异显著,总纤维素消失率增加3.5%。试验结果说明,添加水解酪素具有明显提高纤维素分解菌的活力,负效应是甲烷产气量也适当增加。Two kinds of microorganisms play an important in cellulose-decomposing and on rumen fermentation and methanogenesis for animals and environment. The reaction process of methane gas is not only nutrients consuming but also the primary source of greenhouse gases. The main carbohydrate of rumens is provided by cellulose decomposition bacteria. One key point of this paper is to study the activity of cellulose decomposition bacteria. The methane gas production in vitro was measured by the gas device in rumens fluid extracted from Tibetan sheep through a rumen tube. The disappearance rate of cellulose was analyzed by no textile fiber bags within rumen fluid, the bacterial colony within rumen fluid was counted by anaerobic culture. The results showed that the methane gas production had increased 22. 35% with addition of a certain amount of hydrolyzed casein, Differences were no significant before and after the added hydrolyzed casein （p = 0. 433 ）, and the total disappearance rate of cellulose was 25.8 % a significant 3.5 % increase （p = 0. 043 ）. The results prove that addition of hydrolyzed casein enhances the vitality of cellulose-decomposing. However, the methane gas oroduction is also increased,

关 键 词：藏系绵羊 瘤胃液 体外产气 纤维素消化率 水解酪素 

分 类 号：S816.79[农业科学—饲料科学]