新疆中哈边境地区分离到远东型和西伯利亚型蜱传脑炎病毒  

Isolation of tick-borne encephalitis virus Far-eastern subtype and Siberian subtype in the China-Kazakhstan border area in Xinjiang

作　　者：张桂林[1] 孙响[1] 刘然[1] 郑重[1] 刘晓明[1] 伊小平 

机构地区：[1]新疆军区疾病预防控制中心,乌鲁木齐830011 [2]阿拉山口出入境检验检疫局,博乐833418

出　　处：《中国人兽共患病学报》2017年第4期312-315,共4页Chinese Journal of Zoonoses

基　　金：国家自然科学基金项目(No.U1303104)~~

摘　　要：目的研究新疆中哈边境阿拉套山夏尔西里自然保护区蜱传脑炎疫源地病原的基因型及生物学特征。方法采用布旗法采集蜱,活蜱保存或液氮冻存;采用BALB/c小鼠与BHK-21细胞进行蜱传脑炎病毒分离培养;采用RT-PCR扩增蜱传脑炎病毒远东型FE和西伯利亚型S特异基因片段并测定其序列。结果从新疆中哈边境阿拉套山夏尔西里自然保护区全沟硬蜱和森林革蜱中分离出16株蜱传脑炎病毒株,通过对扩增基因序列比对分析,明确其中13株为远东型,3株为西伯利亚型。结论从新疆中哈边境阿拉套山夏尔西里自然保护区分离到远东型和西伯利亚型蜱传脑炎病毒,该地区为两种亚型病毒共存的蜱传脑炎自然疫源地。To investigate the genotype and the biological characteristics of Tick-borne encephalitis virus （TBEV） in Charles Hilary endemic foci in the China Kazakhstan border area in Xinjiang, ticks were collected by flagging during May to June in 2012 and 2014, and were stored in liquid nitrogen. TBEV strains were isolated from tick samples by inoculating BALB/c mice and BHK-21 cells. The FE gene fragments of TBEV-Far and the S gene fragments of TBEV-Sib were detected by RT-PCR from infected mice brain tissue and BHK cells, and then subjected to sequence alignment. Totally 16 TBEV strains were isolated from Ixodes persulcatus and Dermuceuter silvarum, among 13 strains were Far eastern subtype, three strains were Siberian subtype. It was first time that the TBEV-Sib was isolated in China. The Charles Hilary TBE natural foci were in the China-Kazakhstan border area, and both TBEV-Far and TBEV-Sib co-circulated.

关 键 词：蜱传脑炎病毒 远东型 西伯利亚型 疫源地 中哈边境 

分 类 号：R373.3[医药卫生—病原生物学]