多巴胺D4受体基因多态性与学龄双生子儿童行为问题的关系研究  

Relationship of the functional polymorphism of dopamine D4 receptor gene with behavioral problems in school-age twins

作　　者：周艳娜[1] 李玉玲[1] 

机构地区：[1]内蒙古师范大学生命科学与技术学院,呼和浩特010022

出　　处：《中华行为医学与脑科学杂志》2017年第4期293-299,共7页Chinese Journal of Behavioral Medicine and Brain Science

基　　金：国家自然科学基金项目（30960167,31260265）;内蒙古师范大学研究生科研创新基金资助项目（CXJJS15092）

摘　　要：目的探讨学龄双生子儿童多巴胺D4受体（Dopamine D4 receptor，DRD4）基因多态性与行为问题的关系及作用特点。方法采用Achenbach儿童行为量表对147对6～12岁双生子儿童行为问题进行测评；采用PCR技术，对DRD4基因多态性进行分型；运用广义估计方程模型分析双生子儿童DRD4基因多态性与行为问题的关系及其与儿童性别、年龄、卵型的交互作用。结果（1）DRD4基因多态性与学龄双生子儿童焦虑抑郁、退缩、思维问题、注意力问题、社会化问题、违纪行为、攻击行为的发生及总粗分相关（P〈0．05），与身体不适行为可能也有一定相关性（p=0．067）；除社会化问题外，基因型为4／6型（携带5次及以上重复序列片段的基因型）双生子儿童的行为问题检出率（6．25％-21．25％）均高于其他基因型儿童（0-14．74％），差异具有统计学意义（P〈0．05）；（2）退缩行为与儿童年龄有关（χ^2=4．348，P〈0．05），低年龄段（6～10岁）双生子儿童易发生退缩行为；（3）DRD4基因多态性与儿童年龄的交互作用对退缩行为（χ^2=4．348，P〈0．05）和身体不适（χ^2=5．189，P〈0．05）均有影响，高年龄段对4／6型双生子儿童退缩行为和身体不适行为的发生具有一定的保护作用，11～12岁高年龄段检出率均为0，6—10岁低年龄段二者检出率分别为3．54％和2．65％。结论DRD4基因多态性与学龄双生子儿童行为问题存在相关性；DRD4基因多态性与儿童年龄的交互作用对学龄双生子儿童退缩行为和身体不适的发生有影响。Objective To investigate the correlation and characteristic of the functional polymorphism of the dopamine D4 receptor gene on the behavioral problems in school-age twins. Method The behavioral problems were assessed among a total of 147 twin pairs aged 6-12 years with the Aehenbach Child Behavior Checklist （CBCL）. The DRD4 gene polymorphism was applied for different types with polymerase chain reaction. The generalized estimating equations model was used to analyze the effect of DRD4 gene polymorphism and the interaction of DRD4 gene polymorphism with children age, gender, monozygotic/dizygotic on twins＇ behavioral problems. Results （ 1 ） School-age children＇ s anxiety/depression,withdrawn, thought problems ,attention problems, social problems, delinquent problems, aggressive behavior and total variance mainly were correlated with DRD4 gene polymorphism （P〈0.05）, and physical discomfort might be related with DRD4 gene polymorphism （P= 0.067）. Except social problems, the detection rate of behavioral problems of the twins with 4/6 genotype （Gene type carrying 5 or more repeated sequences） of DRD4 gene （6.25%- 21.25% ）was higher than those with other genotypes （0-14.74%）. （2） Withdrawal was related to children age （χ^2= 4.348, P〈O.05）, and twins aged 6-10 might lead to withdrawal. （3） The interaction of DRD4 gene polymorphism and children age had a significant effect on withdrawal （χ^2= 4.348, P〈0.05 ） and physical discomfort（χ^2= 5.189, P〈0.05 ）, and the elder age （11-12 years） children had a certainly protective effect on the withdrawal and physical discomfort with 4/6 genotype in twins. The detection rates were 3.54% and 2.65% in the younger-age （6-10 years） twins, while all 0 in the elder-age （ 11-12 years） for the withdrawal and the physical discomfort spectively. Conclusions The DRDd gene polymorphism may be correlated with behavioral problems in school-age twins. The interaction of DRD4 polymorphism and children age had a significantl

关 键 词：DRD4基因 学龄儿童 双生子 行为问题 

分 类 号：R179[医药卫生—妇幼卫生保健]