氧化型染发剂遗传毒性的体内外微核试验  

Genotoxicity test of oxidative hair dyes by micronucleus tests in vivo and in vitro

作　　者：徐永俊[1] 石莹[1] 王超[1] 张宏伟[1] 

机构地区：[1]中国疾病预防控制中心环境与健康相关产品安全所,北京100021

出　　处：《环境与健康杂志》2016年第12期1079-1081,共3页Journal of Environment and Health

基　　金：中国疾病预防控制中心环境与健康相关产品安全所中日科技合作项目(200613)

摘　　要：目的探讨体外或体内微核试验在氧化型染发剂遗传毒性评价中的应用。方法使用小鼠骨髓细胞微核试验及中国仓鼠肺细胞(CHL细胞)体外微核试验方法检测染发剂作用于小鼠骨髓细胞及CHL细胞后产生的微核率。结果 6种染发剂在体外微核试验中的微核率高于对照组,差异均有统计学意义(P<0.05,P<0.01),在体内微核试验中与对照组相比,差异均无统计学意义(P>0.05)。结论体外或体内微核试验用于检测染发剂的遗传毒性出现不同结果,有必要进一步探讨遗传毒性的测试组合。Objective To test genotoxicity of six kinds of oxidative hair dyes. Methods In vivo mice bone marrow micronucleus test and in vitro CHL cell micronucleus test were involved. Result In vitro micronucleus test, either of the six kinds of oxidative hair dyes increased the micronucleus formation rate significantly compared with the negative control group（P〈0.05, P〈0.01）. However, no significant differences were observed in the micronucleus formation rates between the six kinds of oxidative hair dyes groups and the negative control group. Conclusion Different results between micronucleus test in vivo and in vitro of oxidative hair dyes show the necessity of integrated panel design of the genotoxicity testing methods.

关 键 词：氧化型染发剂 小鼠 骨髓细胞 中国仓鼠肺细胞 微核率 体内外试验 

分 类 号：R994.6[医药卫生—毒理学]