miR-27a和lnc-LRFN2-2基因多态性与移植肾排斥反应发生风险的相关性研究  

miR-27a rs895819 and Inc-LRFN2-2 rs61516247 polymorphism could increase risk of kidney transplantation rejection

作　　者：王本刚[1] 吕执 徐倩[1] 孙丽萍[1] 杨蕾[1] 刘浩[1] 刘永锋[1] 

机构地区：[1]中国医科大学附属一院普通外科研究所暨器官移植科,沈阳110001

出　　处：《中华器官移植杂志》2016年第12期726-730,共5页Chinese Journal of Organ Transplantation

摘　　要：目的探讨非编码RNA多态性与移植肾排斥反应发生风险的关系。方法以1991—2013年间在中国医科大学附属第一医院器官移植科住院的79例肾移植受者为研究对象，根据术后是否发生排斥反应，分为排斥反应组（26例）和无排斥反应组（53例）。以MALDI-TOF-MS方法进行4个微小RNA（miRNA）基因的4个多态性（miR-27a基因rs895819、miR-365b基因rs121224、miR-4795基因rs1002765、let-7e基因rs8111742）和4个长链非编码RNA（lncRNA）基因的8个多态性（lnc-C6orf132-1基因rs7747696、rs10817938、rs7748341、rs7749023和rs80112640，lnc-LRFN2-2基因rs61516247，lnc-LRFN2-1基因rs1886753，lnc-LRFN2-3基因rs72854760）检测。结果miR-27a基因rs895819多态性的变异基因型其排斥反应的发生风险为野生基因型的11.72倍（P＝0.046，95%置信区间＝1.04～131.74）；lnc-LRFN2-2基因rs61516247多态性的变异AA基因型其排斥反应的发生风险为野生GG基因型的4.87倍（P＝0.047，95%置信区间＝1.02～23.21）。其他3种miRNA多态性及7种lnc-LRFN2-2多态性与移植肾排斥反应的发生风险未见明显相关性（P〉0.05）。结论miR-27a基因rs895819多态性和lnc-LRFN2-2基因rs61516247多态性与肾移植后排斥反应发生风险相关。ObjectiveTo explore whether polymorphisms in non-coding RNA has potential as biomarkers for predicting the risk of kidney transplantation rejection.MethodsA total of 79 patients who had received kidney transplants were recruited from the First Affiliated Hospital of China Medical University and divided into the rejection group （n=26） and non-rejection group （n=53）. Four polymorphisms in miRNA and 8 polymorphisms in lncRNA were detected by MALDI-TOF-MS.ResultsWhen compared with the wild genotype, the mutation genotype in miR-27a rs895819 and lnc-LRFN2-2 rs61516247 had 11.72 and 4.87 folds increased risk of kidney transplantation rejection （P=0.046, OR=1.04-131.74 and P=0.047, 95% CI=1.02-23.21, respectively）. The other three polymorphisms in miRNA and 7 polymorphisms in lncRNA showed no significant associations with transplantation rejection risk （P〉0.05）.ConclusionThe miR-27a rs895819 and lnc-LRFN2-2 rs61516247 polymorphisms were associated with the risk of kidney transplantation rejection.

关 键 词：肾移植 多态性 单核苷酸 移植物排斥 

分 类 号：R699[医药卫生—泌尿科学]