小GTP酶Rac1对肺癌A549细胞凋亡和吞噬作用的影响  

Small GTPase Rac1 regulates apoptosis and phagocytosis in A549 cell line

作　　者：齐晨[1] 彭静静[1] 张淡宜 苏兆亮[1,3] 王胜军[1,2] 许化溪[1] QI Chen PENG Jing-jing ZHANG Dan-yi SU Zhao-liang WANG Sheng-jun XU Hua-xi(Department of Immunology, School of Medicine, Jiangsn University, Zbenjiang Jiangsu 212013 Department of Laboratory Medicine, the Affiliated People＇s Hospital of Jiangsu University, Zhenjiang Jiangsu 212002 the Central Laboratory, the Fourth Affiliated Hospital of Jiangsu University, Zhenjiang Jiangsu 212001, China)

机构地区：[1]江苏大学医学院免疫学系,江苏镇江212013 [2]江苏大学附属人民医院医学检验科,江苏镇江212002 [3]江苏大学附属第四医院中心实验室,江苏镇江212001

出　　处：《江苏大学学报（医学版）》2017年第2期93-97,共5页Journal of Jiangsu University：Medicine Edition

基　　金：国家自然科学基金资助项目(81671567);江苏省重点研发计划项目(BE2016716);江苏省高校自然科学研究重大项目(16KJA320005);江苏省博士后基金资助项目(1601002C)

摘　　要：目的:分析肺癌A549细胞Rac1的表达水平及其对细胞吞噬功能的影响。方法:采用姜黄素诱导A549细胞凋亡;采用流式细胞术检测Rac1抑制剂对A549细胞凋亡的影响;将A549细胞与同系凋亡细胞共培养,应用荧光显微镜技术观察Rac1下调对A549细胞清除凋亡细胞能力的影响。用qRT-PCR检测细胞Rac1 mRNA表达水平。结果:流式双染和Hoechst染色荧光显微镜观察结果显示,Rac1抑制剂能够明显增强姜黄素诱导的A549细胞凋亡,凋亡细胞率相对于未加入Rac1抑制剂时显著上升,且呈现与Rac1抑制剂的剂量依赖特性。此外,Rac1抑制剂能明显下调A549细胞的吞噬能力。结论:Rac1可抑制肺癌A549细胞的凋亡,增强其吞噬功能。Objective：To investigate the Rac1 expression level,and analyze the effect of Rac1 on the clearance of apoptotic cells and regulation of phagocytosis in A549 cell line.Methods：A549 cell was selected as the cell model.The curcumin was used to induce apoptosis,and the A459 cells and apoptotic cells co-culture method was used for analyzing the relationship between the down-regulation of Rac1 function and the ability of apoptotic cell clearance.The qRT-PCR was used to detect the expression level of Rac1 mRNA.Flow cytometry and fluorescence microscopy techniques were used to observe and analyze the apoptotic cells and phagocytic capacity.Results：Flow cytometry and Hoechst staining showed that the apoptotic rate of airway epithelial cells was increased in the presence of Rac1 inhibitor,and this phenomenon was dose dependent.Flow cytometry analysis showed that the Rac1 inhibitor could markedly down-regulate the phagocytic ability in A549 airway epithelial cells.Conclusion：The expression of Rac1 plays a negative role in the regulation of A549 cell apoptosis,but has a significantly effect on the phagocytosis of epithelial cells.

关 键 词：肺癌A549细胞 Ras相关C3肉毒素底物1 细胞凋亡 细胞吞噬 

分 类 号：R392.12[医药卫生—免疫学]