银杏叶片对高砷煤烘玉米粉致大鼠肝损害的干预作用  

Effect of Ginkgo biloba on liver injury of arsenic poisoning rats caused by corn flour baked by high-arsenic coal

作　　者：姚茂琳[1] 张爱华[1] 于春[1] 徐玉艳[1] 胡勇[1] 

机构地区：[1]贵州医科大学环境污染与疾病监控教育部重点实验室、卫生毒理学教研室,贵阳550025

出　　处：《中华地方病学杂志》2017年第5期333-337,共5页Chinese Journal of Endemiology

基　　金：国家自然科学基金（81172603、81430077）

摘　　要：目的 探讨银杏叶片对燃煤砷污染致大鼠肝损害的干预效果及其机制，为砷中毒的防治提供实验依据。方法 选择40只健康断乳Wistar大鼠，按体重采用随机数字表法分为正常对照A组、砷中毒组、正常对照B组、砷中毒自然恢复组和银杏叶片治疗组，每组8只，雌雄各半。以高砷煤烘烤的玉米粉为主要原料制备大鼠含砷饲料。正常对照A组喂饲正常普通饲料3.0个月；砷中毒组喂饲含砷饲料（100 mg／kg）3.0个月；正常对照B组喂饲正常普通饲料4.5个月；砷中毒自然恢复组喂饲含砷饲料（100 mg／kg）3.0个月后，喂饲正常普通饲料1.5个月；银杏叶片治疗组大鼠喂饲含砷饲料（100 mg／kg）3.0个月后，给予银杏叶片溶液灌胃（25 mg／kg，6 d／周）1.5个月，治疗期间喂饲正常普通饲料。实验终期采集大鼠尿液、血液和肝脏组织，检测尿砷和肝脏砷含量，肝功能指标丙氨酸氨基转换酶（ALT）、天冬氨酸氨基转换酶（AST）、总胆汁酸（TBA）、γ-谷氨酰转肽酶（GGT）、谷胱甘肽硫转移酶（GSTs），以及肝脏氧化损伤指标超氧化物歧化酶（SOD）、谷胱甘肽过氧化物酶（GPx）活性，巯基（-SH）水平和丙二醛（MDA）含量。结果 砷中毒组大鼠尿砷和肝脏砷含量［几何均数（Ｇ）分别为2 991.24 μg／g Cr，4.29 μg／g］均明显高于正常对照A组（91.59 μg／g Cr，1.00 μg／g，P均 ＜ 0.05）；砷中毒自然恢复组和银杏叶片治疗组尿砷（467.39、334.48 μg／g Cr）和肝脏砷含量（3.15、1.88 μg／g）明显高于正常对照B组（99.54 μg／g Cr，0.85 μg／g，P均 ＜ 0.05）；砷中毒自然恢复组的尿砷、银杏叶片治疗组的尿砷和肝脏砷均显著低于砷中毒组（P均 ＜ 0.05）。砷中毒组大鼠血清AST、TBA、GGT、GSTs分别为（212.88 ± 29.76）U／L，（19.19 ± 4.33）μmol／L，（1.73 ± 0.50）、（196.21 ± 47.38）U／L，均显著高于正常对Objective To explore the effects and the possible mechanism of Gingko biloba on liver injury due to arsenic poisoning in rats, and to provide experimental evidence for prevention and treatment of arsenic poisoning. Methods The corn powder baked by high arsenic coal was served as the main raw material to make feed containing arsenic. Forty healthy Wistar rats were randomly divided into 5 groups according to their body weights, including control group A, arsenic poisoning group, control group B, natural recovery group and Ginkgo biloba treatment group, eight rats in each group, half male and half female. The control group A rats were fed with normal diet ad libitum for 3.0 months; the arsenic poisoning group rats were freely given feed containing arsenic （100 mg/kg） for 3.0 months; the control group B rats were fed with normal diet ad libitum for 4.5 months; the natural recovery group rats were freely given arsenic （100 mg/kg） feed for 3.0 months, and then given a normal diet for 1.5 months; Ginkgo biloba treatment rats ingested arsenic feed for 3.0 months, and then give Ginkgo biloba solution （25 mg/kg） orally, 6 d/week for 1.5 months, then back to normal diet. The content of arsenic in urine, liver, as well as the liver function indices [alanine aminotransferase （ALT）, aspartate transaminase （AST）, total bile acids （TBA）, gamma glutamyl aminopeptidase （GGT）, glutathione S-transferase （GSTs）] and the oxidative stress indexes [superoxide dismutase （SOD）, glutathione peroxidase （GPx）, thiol （-SH）, malondialdehyde （MDA）] of liver homogenate, were measured. Results The arsenic content of urine and liver （geometric mean） of the rats in arsenic poisoning group （2 991.24 μg/g Cr, 4.29 μg/g） were significantly higher than those in control group A （91.59 μg/g Cr, 1.00 μg/g）. Urinary arsenic and liver arsenic levels of rats in natural recovery and Ginkgo biloba treatment groups （467.39, 334.48 μg/g Cr; 3.15, 1.88 μg/g） were higher than those in c

关 键 词：砷中毒 银杏叶片 大鼠 肝损害 氧化损伤 

分 类 号：R285.5[医药卫生—中药学]