PIWI蛋白家族基因在绵羊生殖腺中的表达研究  

Study on gene expression of PIWI protein family in the sheep gonad

作　　者：特日格勒[1] 付绍印[1] 李虎山[2] 王标[1] 何小龙[1] 祁云霞[1] 达赖[1] 刘永斌[1] 

机构地区：[1]内蒙古农牧业科学院,呼和浩特010031 [2]巴彦淖尔市家畜改良站,内蒙古巴彦淖尔015000

出　　处：《黑龙江畜牧兽医》2017年第5期100-103,共4页Heilongjiang Animal Science And veterinary Medicine

基　　金：国家自然科学基金项目(31360542);国家肉羊产业技术体系建设项目(CARS-39);"十二五"国家科技支撑计划项目(2011BAD28B05);内蒙古自治区农牧业科学院创新基金项目(2015CXJJM02)

摘　　要：为了研究PIWI基因对绵羊生殖细胞发育的影响,试验采用同源基因克隆的方法,以绵羊睾丸、卵巢RNA为模板,通过PCR扩增,半定量PIWI基因家族在苏尼特羊、小尾寒羊生殖腺的表达。结果表明:公羊PIWI蛋白家族3个成员mRNA的相对表达水平均极显著高于母羊(P<0.01),PIWI1、PIWI2、PIWI4在高繁小尾寒羊母羊、低繁苏尼特母羊均只有微弱表达,同性别高繁小尾寒羊、低繁苏尼特羊mRNA相对表达水平差异不显著(P>0.05)。To investigate effect of PIWI gene family on the development of germ cells of sheep, the PIWI gene family were semi quantitative analysis in the testis and ovary tissues of Sunite sheep and small tail Han sheep by using homology cloning method. The qRT - PCR analysis indicated that the mRNA relative expression levels of three members of PIWI protein family in rams were significantly higher than that of the ewes （P 〈0.01 ）, while the PIWI1, PIWI2 and P1WI4 genes were only weakly expressed in highly prolific small Tail Han sheep and lower prolific Sunit ewes; the mRNA relative expression level have no significant difference （P〉0.05 ） to the same sex in the highly prolific small Tail Han sheep and lower prolific Sunit.

关 键 词：PIWI 小尾寒羊 苏尼特羊 繁殖力 生殖腺 

分 类 号：S826[农业科学—畜牧学] Q786[农业科学—畜牧兽医]