重庆大巴山地区中蜂遗传多样性分析  

Genetic diversity of Apis cerana cerana Fabricius in the Daba mountain of Chongqing

作　　者：姬聪慧[1] 戴荣国[1] 罗文华[1] 任勤[1] 刘佳霖[1] 王瑞生[1] 曹联飞[2] 

机构地区：[1]重庆市畜牧科学院,重庆402460 [2]浙江省农业科学院,杭州310021

出　　处：《黑龙江畜牧兽医》2017年第5期232-233,238,299,共4页Heilongjiang Animal Science And veterinary Medicine

基　　金：现代农业产业技术体系蜂产业技术体系项目(11201);重庆市城口县科委科技计划资助项目"城口中蜂种质资源保护与利用研究"(13503);"蜜蜂养殖合作社+公司"产业化模式示范与推广项目(14460)

摘　　要：为了保护大巴山地区的中蜂资源,试验采集重庆市城口县111只中蜂样本,采用线粒体DNA tRNAleu-COⅡ基因测序的方法对大巴山中蜂进行遗传多样性分析。结果表明:大巴山地区111只中蜂样本中共检测到单倍型21种,群体的单倍型多样度(Hd)为0.859 0,平均核苷酸差异数(K)为2.482,核苷酸多样度(Pi)为0.006 01,其中有5个单倍型属于首次发现的单倍型。说明大巴山地区中蜂的遗传多样性较高,具有独特的遗传背景,其开发潜力,尤其是保种和育种价值很大。To investigate the Apis eerana eerana Fabrieius resources in Daba Mountain, 111 Apis eerana samples were collected from the Cheng - kou County of Chongqing, and then the genetic diversity was analyzed based on mitoehondrial tRNAleu - COⅡ sequences. The results showed that there were 21 haplotypes among the 111 samples, and the haplotypc diversity（Hd） was O. 859 0 ; the average number of nueleotide differences（K） was 2. 482 ,the nucleotide diversity（Pi） was 0. 006 01. It was also found that, 5 of the 21 haplotypes were the first to be found. The results indicated that Apis cerana cerana in the Daba Mountain had relatively higher genetic diversity and exhbited its own unique genetic background. Therefore, the Daba mountain has a potential for the developmentand the conservation of species and breeding.

关 键 词：大巴山 中蜂 遗传多样性 线粒体tRNAleu—COⅡ 保种价值 线粒体DNA 

分 类 号：S894.13[农业科学—特种经济动物饲养] Q346[农业科学—畜牧兽医]