牛大力多糖对小鼠脾细胞增殖及分泌细胞因子的影响  

Effects of Polysaccharides from Millettia speciosa Champ on Proliferation of Spleen Lymphocyte and Secretion of Cytokine in Mice

作　　者：王柳萍[1] 沈茂杰[2] 杨斌[3] 莫凯[4] 李良波[2] 钟霞军[5] 黄荣韶[2] 

机构地区：[1]广西中医药大学药学院,南宁530001 [2]广西大学农学院,南宁530003 [3]广西医科大学药学院,南宁530022 [4]南宁市第一人民医院药剂科,南宁530003 [5]广西中医药大学医药会展中心,南宁530001

出　　处：《医药导报》2017年第5期480-483,共4页Herald of Medicine

基　　金：广西自然科学基金资助项目(2012GXNSFAA276034);玉林市科学研究与技术开发项目(玉市科[2014]28);南宁市科学研究与技术开发计划项目(20133032-3)

摘　　要：目的观察牛大力多糖对小鼠脾细胞增殖及分泌细胞因子的影响,探讨其免疫调节的作用机制。方法采用噻唑蓝(MTT)法检测牛大力多糖对刀豆蛋白A(Con A)刺激的小鼠T淋巴细胞增殖的影响;采用酶联免疫吸附(ELISA)法测定肿瘤坏死因子(TNF-α)、白细胞介素-6(IL-6)、前列腺素E_2(PGE_2)的含量。结果牛大力多糖浓度在50~200μg·mL^(-1)范围内能增强Con A诱导的小鼠T淋巴细胞的增殖作用;并对TNF-α和IL-6的产生有促进作用,对PGE_2有抑制作用。结论牛大力多糖能促进小鼠脾细胞的增殖,通过调节细胞因子发挥免疫增强作用。Objective To explore the mechanism of immunoregulation by investigating the effects of polysaccharides from Millettia speciosa Champ （MSC） on proliferation of spleen lymphocyte and secretion of cytokine in mice.Methods The effects of MSC polysaccharides on Con A-induced spleen T lymphocyte proliferation were determined by MTT.The contents of TNF-α, IL-6 and PGE2 were determined by ELISA.Results The Con A-induced T lymphocyte proliferation was significantly increased by MSC polysaccharides at the concentrations from 50 to 200 μg·mL-1.Coupled with TNF-α and IL-6 were significantly increased while PGE2 was significantly decreased.Conclusion MSC polysaccharides could increase proliferation of spleen lymphocyte and enhance the immune responses by increasing the secretion of TNF-α and IL-6 in mice.

关 键 词：牛大力多糖 脾细胞增殖 细胞因子 

分 类 号：R282.71[医药卫生—中药学] R285.5[医药卫生—中医学]