启膈散对食管癌EC9706细胞株抑制树突状细胞成熟的影响  

The effect of Qige powder on the mature dendritic cells inhibited by the esophageal cancer EC9706 cells

作　　者：吴耀松[1] 任闪闪[1] 陈玉龙[1] 尹素改[1] 金华彬 王朋辉[1] 

机构地区：[1]河南中医药大学,河南郑州450008

出　　处：《时珍国医国药》2017年第4期769-772,共4页Lishizhen Medicine and Materia Medica Research

基　　金：国家自然科学基金(No.81373535;No.81173177)

摘　　要：目的观察启膈散对食管癌细胞干预树突状细胞成熟的影响。方法制备条件培养基,用Fieoll密度梯度离心法分离外周血单个核细胞,体外诱导培养树突状细胞(Dendritic Cells,DCs),流式细胞术检测DCs表面标志物,MTT法检测淋巴细胞细胞增殖;LDH释放法检测细胞毒T淋巴细胞(cytotoxic T lymphocyte,CTL)杀伤力;ELISA检测细胞因子分泌,Western blot检测STAT3蛋白表达。结果食管癌EC9706细胞条件培养基可以减少外周血单核细胞诱导的树突状细胞表达CD80、CD86、CD1a,抑制DCs刺激淋巴细胞增殖和减弱CTL的杀伤力,减少IL^(-1)2分泌,增加STAT3蛋白表达和磷酸化;与肿瘤条件培养基相比,启膈散条件培养基组DCs CD80、CD86、CD1a增加,淋巴细胞的增殖能力、CTL杀伤作用增强,IL^(-1)2分泌增加,STAT3蛋白表达和磷酸化减少。结论启膈散可以通过STAT3信号通路减弱食管癌细胞上清对树突状细胞成熟的影响。Objective To observe the effect of Qige powder on the mature of dendritic cells intervened by the esophageal cancer EC9706 ceils. Methods Conditioned medium was prepared and the mononuclear cells in peripheral blood were separated by Fie- oll density gradient centrifugation; The dendritic ceils were induced in vitro; The surface markers of DCs were detected by FCM and the proliferation of the lymphocyte cells by MTI＂ and the lethality of cytotoxic T cell by LDH release assays and the secretion levels of cytokine by ELISA and the expression of STAT3 by Western blot. Results Conditioned medium from Esophageal EC9706 cells can suppress the mature of dendritic ceils from mononuclear cells in peripheral blood ; Inhibit the proliferation of lymphocyte by stimulating DCs and the lethality of cytotoxic T cell; Reduce the secretion of IL - 12 and increase the expression and phospho- rylation of STAT3 in DCs ; Compared with the group of conditioned medium from Esophageal cells, Qige powder increased the lev- el of DCs CD80, CD86, CD1 a and the proliferation of the lymphocyte cells and the lethality of CTL and the IL - 12 secretion of DCs ; Decreased the expression and phosphorylation of STAT3. Conclusion Qige powder can lessened the influence of the esoph- ageal cancer cells conditioned medium on the mature of dendritic cells by STAT3 signal pathway.

关 键 词：食管癌 树突状细胞 启膈散 STAT3 

分 类 号：R285.5[医药卫生—中药学]