建中理劳汤含药血清对TGF-β1诱导大鼠系膜细胞HBZY-1纤维化的作用机制的研究  

Effects of Jianzhonglilao Decoction-mediated Serum on TGF-β1-induced Fibrosis of the Rat Mesangial Cell Line HBZY-1

作　　者：沈会[1,2] 陶汉华[1] 张诏[1] 展照双[1] 朱炜楷 赵妍妍[2] 

机构地区：[1]山东中医药大学,山东济南250355 [2]大连医科大学附属第一医院,辽宁大连116011

出　　处：《时珍国医国药》2017年第4期783-787,共5页Lishizhen Medicine and Materia Medica Research

基　　金：国家自然科学基金面上项目(81273635)

摘　　要：目的通过体外细胞实验探讨建中理劳汤含药血清对TGF-β1诱导大鼠系膜细胞HBZY-1纤维化过程中TGF-β/Smad信号通路的调控作用。方法采用PCR、Western Blot方法观察建中理劳汤含药血清对TGF-β1诱导大鼠系膜细胞HBZY-1纤维化过程中Smad-2、Smad-3、Smad-7 mRNA及蛋白表达的影响;通过Western Blot方法检测5.5μg/L的TGF-β1诱导HBZY-1细胞不同时间p-Smad2、p-Smad3蛋白的表达影响;Western Blot检测建中理劳汤大鼠含药血清对TGF-β1诱导30min后的HBZY-1细胞p-Smad2、p-Smad3蛋白的表达影响。结果与正常组相比,TGF-β1组中p-Smad2、p-Smad3蛋白表达显著升高,Smad-7表达明显降低(P<0.05);而与TGF-β1组相比,加入建中理劳汤含药血清后,p-Smad2、p-Smad3蛋白表达显著降低,Smad-7表达明显升高(P<0.05)。结论建中理劳汤含药血清对TGF-β1诱导大鼠系膜细胞HBZY-1纤维化过程中TGF-β/Smad信号通路起调控作用,可能是其抗纤维化形成的机制之一。Objective To investigate the regulation of Jianzhonglilao Decoction - mediated serum on the TGF - β/Smad signaling pathway in the process of TGF - β1 - induced fibrosis of the rat mesangial cell line HBZY - 1 through in vitro cell experiments. Methods The effects of Jianzhonglilao Decoction - mediated serum on the mRNA and protein level of Smad - 2, Smad - 3 and Smad -7 in the process of TGF - β1 - induced fibrosis of the rat mesangial cell line HBZY - 1 were assessed by PCR and Western blot assay. The changes of p - Smad2 and p - Smad3 protein level were examined by Western blot assay in HBZY - 1 cells in- duced by TGF -β1 （5.5μg/L） for different time points. The effects of Jianzhonglilao Decoction - mediated serum on the expres- sion of p - Smad2 and p - Smad3 proteins were determined by in HBZY - 1 cells induced by TGF - β1 for 30 min. Results The expression of p - Smad2 and p - Smad3 protein significantly increased, whereas Smad - 7 expression significantly decreased in HBZY- 1 cells treated with TGF- β1 compared with that in control cells. However,the expression of p- Smad2 and p -Smad3 protein significantly decreased, whereas Smad -7 expression significantly increased in HBZY - 1 ceils treated with Jianzhonglilao Decoction- mediated serum compared with that in HBZY - 1 cells treated with TGF - β1. Conclusion The regulation of Jianzhonglilao Decoction - mediated serum on the TGF -β/Smad signaling pathway in the process of TGF - β1 - induced fibrosis of the rat mesangial cell line HBZY - 1 may be one of its anti - fibrosis formation mechanisms.

关 键 词：建中理劳汤 大鼠系膜细胞 TGF-Β1 纤维化 TGF-Β/SMAD信号通路 

分 类 号：R285.5[医药卫生—中药学]