瑞香狼毒药材中伞形花内酯和狼毒色原酮含量的高效液相色谱法测定  

Simultaneous Determination of Umbelliferone and Chamechromone in Stellera chamaejasme by RP-HPLC

作　　者：魏丽[1] 王静静[2] 杨艳梅[1] 赵丽艳[1] 张万明[1] 张丹参[1] 

机构地区：[1]河北北方学院药学系,河北张家口075000 [2]河北北方学院附属第一医院,河北张家口075000

出　　处：《时珍国医国药》2017年第4期815-817,共3页Lishizhen Medicine and Materia Medica Research

基　　金：国家自然科学基金(No.81274005);河北北方学院基金资助项目(No.GZ1405)

摘　　要：目的建立RP-HPLC法测定瑞香狼毒药材中伞形花内酯和狼毒色原酮的含量。方法采用反相高效液相色谱法,PDA检测器,选用Hypersil GOLDaQ-C_(18)(250mm×4.6mm,5μm),色谱柱;流动相为乙腈(A)-0.1%磷酸水溶液(B),进行梯度洗脱;检测波长伞形花内酯为327 nm,狼毒色原酮为297nm;流速1.0ml/min;进样量10μl,柱温25℃。结果2种活性成分在13 min内实现良好分离,伞形花内酯、狼毒色原酮的线性范围分别为1.22~19.6μg·ml^(-1)(r=0.9998)、20.0~320μg·ml^(-1)(r=0.9997),平均回收率分别为100.5%,101.8%,RSD分别为1.2%,1.5%。13批不同产地瑞香狼毒药材中伞形花内酯的含量范围为:0~0.57mg/g;狼毒色原酮的含量范围6.46~30.92mg/g。结论该方法操作简便、重现性好、结果准确可靠,可用于中药瑞香狼毒的质量控制。Objective A RP - HPLC method was established to detect the content of umbelliferone and chamechromone in 13 bat- ches SteUera chamaejasme from different areas in this paper. Methods A Hypersil Gold aQ - C18 （4.6 mmx 150 mm, 5txm） column was selected in this experiment; the mobile phase was acetonitrile （A） -0.1% phosphate aqueous solution （B） with gradient elution; the wavelength was umbelliferone 327 nm, chamechromone 297nm; the flow rate was 1.0 mL/min; the column temperature was 25℃. Results The 2 constituents were separated within 13min. The linear ranges of umbelliferone and cha- mechromone were 38.5 - 166.7 ug mL-1 （ r = 0. 9998 ） , 15.9 - 254.5 ug ml - l （ r = 0. 9997 ） respectively. The average re- coveries were 100.5% ,101.8% respectively. The RSD were 1.2% , 1.5% respectively. The content range of umbelliferone in Stellera chamaejasme was： 0 -0. 57mg/g; the content range of chamechromone in Stellera chamaejasme was 6.46 -30.92mg/g. Conclusion The method is simple, accurate and reliable, and could be used for quality control of traditional Chinese medicine Stellera chamaejasme.

关 键 词：高效液相色谱 瑞香狼毒 伞形花内酯 狼毒色原酮 

分 类 号：R284.2[医药卫生—中药学]