白屈菜中总生物碱含量的酸性染料比色法测定  

Determination of total alkaloids in chelidonii herba by acid dye colorimetry

作　　者：姜华[1] 李军[1] 徐强[1] 冯晴爽 付兴兴 

机构地区：[1]河南科技大学化工与制药学院,河南洛阳471023

出　　处：《时珍国医国药》2017年第4期854-856,共3页Lishizhen Medicine and Materia Medica Research

基　　金：河南科技大学博士科研启动基金项目(No.13480058)

摘　　要：目的测定白屈菜中总生物碱的含量,为其质量的控制提供实验依据。方法以白屈菜红碱为标准品,采用酸性染料比色法测定白屈菜总生物碱的含量。结果白屈菜红碱在0.016~0.096 mg.ml^(-1)范围内,回归方程为Y=9.8661X+0.025,r=0.9991,吸光度与浓度具有良好的线性关系,平均回收率分别99.93%,RSD=1.79%。结论该方法准确度好,有一定的专属性,为白屈菜质量的控制提供实验依据。Objective To determine total alkaloids in chelidonii herba, which can be used for quality control. Methods Chel- erythrine was used as a control standard. The contents of total alkaloids were determined by acid dye colorimetry. Results The linear range of chelerythrine was 0. 016 -0. 096 mg. ml-1 , the regression equation was Y = 9. 8661X ＋ 0. 025, r = 0. 9991. The average recovery was 99.93 % ,and RSD was 1.79 %. Conclusion The method is accurate with good reproducibility, which can be used for quality control.

关 键 词：白屈菜 总生物碱 酸性染料比色法 

分 类 号：R284.1[医药卫生—中药学]