电针对脊髓损伤大鼠Nogo-A、NgR mRNA及蛋白表达影响的实验研究  

Experimental study of electroacupuncture on mRNA and protein expression of Nogo-A and NgR in SCI rats

作　　者：闵友江[1] 张辉[1] 肖伟平[1] 闵志云[1] 裴佳[1] 姚海华[1] 程立红[1] 

机构地区：[1]江西中医药大学附属医院,江西南昌330006

出　　处：《时珍国医国药》2017年第4期995-998,共4页Lishizhen Medicine and Materia Medica Research

基　　金：国家自然科学基金(No.81360562)

摘　　要：目的探讨电针治疗对大鼠脊髓损伤后Nogo-A、NgR mRNA及蛋白表达的影响。方法 72只SD雄性大鼠随机分成假手术组(A,n=8)和造模组(n=64),造模组大鼠采用改良Allen’s重物打击法制作脊髓损伤大鼠模型,造模成功后存活56只大鼠,随机分为模型组(B,n=14)、电针组(C,n=14)、药物组(D,n=14)、电针+药物组(E,n=14),并分别于治疗后第14d提取损伤处脊髓组织,采用实时荧光定量PCR、原位杂交、Western blot方法分别检测Nogo-A、NgR mRNA及蛋白的表达,采用BBB方法评估大鼠后肢活动功能。结果脊髓损伤后,电针治疗可以显著降低脊髓组织中Nogo-A、NgR mRNA及蛋白的表达,与模型组和药物组比较均具显著性差异(P<0.01);与电针+药物组之间比较无明显差异(P>0.05)。结论电针治疗对大鼠脊髓损伤后Nogo-A和NgR的表达具有明显的抑制作用,这可能是电针治疗脊髓损伤的关键机制之一。Objective To investigate the effect of electroacupuncture （EA） on mRNA and protein expression of Nogo - A and NgR in rats after SCI. Methods A total of 72 Sprague Dawley rats were randomly assigned to sham operation group （ Group A, n = 8） and model- building group （n = 64）. In model groups, Allen＇s method was used to make SCI rats model. 56 model rats were further subdivided into four subgroups（ model control group （Group B） , EA group （Group C） , medicine group（ Group D） and EA ＋ medicine group（ Group E） ）according to treatment. After 14 days, injuried spinal cord tissue was extracted for detec- ting. mRNA and protein expression of Nogo - A and NgR were detected by real - time fluorescent quantitative PCR, in situ hy- bridization and Western blot respectively. The bindlimb motor function in rats was evaluated by using Basso - Beattie - Bresnahan assessment methods. Results Compared with model group and medicine group, EA treatment can significantly reduce mRNA and protein expression of Nogo- A and NgR in spinal cord tissues after treating 14d（P 〈 0.01 ） ; compared with EA plus medicine group, there was no significant difference in the EA groups （ P 〉 0.05 ）. Conclusion EA treatment has obvious inhibitory effect on mRNA and protein expression of Nogo - A and NgR in rats after SCI, which may be one of the key mechanism for EA in trea- ting SCI.

关 键 词：脊髓损伤 电针 神经再生 Nogo/NgR信号通路 

分 类 号：R285.5[医药卫生—中药学]