胃炎1号方对胃癌前病变大鼠胃黏膜上皮细胞缺氧及缺氧耐受的影响  

Effects of No.1 Weiyan Decoction on hypoxia and hypoxic tolerance in gastric mucosal epithelial cells in rats with gastric precancerous lesion

作　　者：张成哲 卓俊城 蔡甜甜[1] 赵自明 潘华峰[1] 陈玉兴 曾晓会 黄雪君 姚楠 蔡大可 

机构地区：[1]广州中医药大学,广东广州510405 [2]广东省中医药工程技术研究院,广东广州510095

出　　处：《中成药》2017年第5期896-901,共6页Chinese Traditional Patent Medicine

基　　金：国家自然科学基金项目(81273739;81473620)

摘　　要：目的研究胃炎1号方(党参、白芍、蒲公英等)对胃癌前病变的大鼠胃黏膜上皮细胞缺氧及缺氧耐受的影响。方法 40只大鼠随机分为空白组、模型组、维酶素组和胃炎1号方组。除空白组外,其他组大鼠采用N-甲基-N'-硝基-N-亚硝基胍(MNNG)和小承气汤建立癌前病变大鼠模型。给药10周后,观察胃黏膜上皮微血管超微结构,测定胃黏膜上皮缺氧诱导因子-1α(Hif1a)mRNA、HIF-1α、血管内皮细胞生长因子(VEGF)蛋白的表达。结果与空白组相比,模型组胃黏膜上皮微血管数量减少,管腔严重狭窄或扩张,内壁不光滑,有粘附,血管内皮细胞肿胀;Hif1a mRNA表达有升高趋势,HIF-1α、VEGF蛋白呈异常表达;与模型组相比,胃炎1号方组改善了胃癌前病变大鼠胃黏膜血管病变,显著降低Hif1a mRNA、HIF-1α、VEGF蛋白的表达。结论胃炎1号方可改善胃癌前病变大鼠胃黏膜上皮缺氧及缺氧耐受,并促进血管新生。AIM To study the effects of No. 1 Weiyan Decoction （ Codonopsis Radix, Paeoniae Radix alba, Taraxaci Herba, etc. ） on hypoxia and hypoxic tolerance in gastric mucosal epithelial cells in rats with precancerous lesion of gastric cancer （PLGC）. METHODS Forty rats were randomly divided into normal, model, Vitacoenzyme Tablets and No. 1 Weiyan Decoction groups. Except for the normal group, the rats in the other groups were given with N-methyl-N＇-nitro-N-nitrosoguanidine and Little Chengqi Decoction to establish a PLGC rat model. Ten weeks after the administration, the gastric mucosal epithelial mierovascular ultrastrueture was observed, the expres- sions of Hifla mRNA, HIF-1 α protein and VEGF protein in gastric mucosal epithelium were detected. RESULTS Compared with the normal group, the number of microvasculatures in gastric mucosal epithelium was decreased ; lumen was severely stenosed or expansive; inner wall was not smooth and had adhesion; vascular endothelial cells were swollen; the expression of Hifla mRNA showed an increasing trend; the expressions of HIF-lct protein and VEGF protein were anomalous in the model group. Compared with the model group, the gastric mucosal vascular lesion of PLGC rats was improved, and the expressions of Hifla mRNA, HIF-1α protein and VEGF protein were significantly decreased in the No. 1 Weiyan Decoction group. CONCLUSION No. 1 Weiyan Decoction can improve hypoxia and hypoxia tolerance, and induce

关 键 词：胃炎1号方 胃癌前病变 胃黏膜上皮 微血管超微结构 缺氧诱导因子-1α(HIF-1α) 血管内皮生长因子(VEGF) 

分 类 号：R285.5[医药卫生—中药学]