黄芪、水蛭有效成分对大鼠肾小球系膜细胞凋亡的影响  

Influences of Astragalus and Hurido effective components on apoptosis of rat glomerular mesangial cells

作　　者：姜梦真 任惠娟 徐蕾[1] 任现志[1] 

机构地区：[1]南京中医药大学,江苏南京210029 [2]江宁区中医院儿科,江苏南京211100

出　　处：《中成药》2017年第5期902-906,共5页Chinese Traditional Patent Medicine

基　　金：国家自然科学基金面上项目(81173298)

摘　　要：目的探讨黄芪、水蛭有效成分对大鼠肾小球系膜细胞凋亡的影响。方法脂多糖诱导大鼠系膜细胞增殖,黄芪(黄芪多糖、黄芪甲苷、毛蕊异黄酮)、水蛭(水蛭素)进行干预,24 h后收集细胞,采用4',6-二脒基-2-苯基吲哚(4',6-diamidino-2-phenylindole,DAPI)染色法观察细胞核的变化,链霉亲和素-生物素复合物测定Bcl-2的定性及定量表达。结果黄芪、水蛭有效成分均能诱导大鼠肾小球系膜细胞凋亡,并能降低Bcl-2的表达。结论黄芪、水蛭有效成分可通过下调Bcl-2表达来诱导系膜细胞凋亡,可用于治疗系膜增生性肾小球肾炎。AIM To investigate the influences of Astragalus and Hurido effective components on apoptosis of rat glomerular mesangial cells. METHODS The proliferation of rat mesangial cells was induced by LPS and then intervened with Astragalus （ cells were collected after 24 astragalus polysaccharide , astragaloside IV, calycosin） and Hurido （hirudin）. The h. 4＇, 6-Diamidino-2-phenylindole （DAPI） staining was used to observe cell nuclear changes. Quantitative and qualitative expressions of Bcl-2 were detected by streptavidin-biotin complex （SABC）. RESULTS Astragalus and Hurido effective components could induce the apoptosis of rat glomerular mesangial ceils, and decrease the expression of Bcl-2. CONCLUSION Astragalus and Hurido effective components can induce the mesangial cell apoptosis by down-regulating Bcl-2 expression, which can be used for the treatment of mesangial proliferative glomerulonephritis.

关 键 词：黄芪 水蛭 有效成分 细胞凋亡 系膜细胞 BCL-2 

分 类 号：R285.5[医药卫生—中药学]