RP-HPLC法同时测定猴头菌片中5种核苷  

Simultaneous determination of five nucleosides in Houtoujun Tablets by RP-HPLC

作　　者：唐海明[1,2] 王德勤[1] 张慧晔[1] 

机构地区：[1]广州白云山和记黄埔中药有限公司,广东广州510515 [2]广州白云山和记黄埔中药(亳州)有限公司,安徽亳州236800

出　　处：《中成药》2017年第5期964-967,共4页Chinese Traditional Patent Medicine

基　　金：广东省岭南药用植物资源保护与利用企业重点实验室(产学研)培育基地(2012A061600005);南药资源综合开发国际合作重点研究室(国中医药科技函[2010]204)

摘　　要：目的建立RP-HPLC法同时测定猴头菌片(猴头菌菌丝体)中5种核苷的含有量。方法该药物水提物的分析采用Zorbax SB-Aq C_(18)色谱柱(250 mm×4.6 mm,5μm);以甲醇-0.1%磷酸为流动相,梯度洗脱;体积流量0.5 m L/min;柱温26℃;检测波长260 nm。结果胞苷、腺嘌呤、尿苷、腺苷、鸟苷分别在0.007~0.169、0.005~0.115、0.032~0.797、0.032~0.792、0.004~0.103μg范围内呈良好的线性关系,平均加样回收率(n=6)分别为99.4%(RSD=2.0%)、98.3%(RSD=1.8%)、98.0%(RSD=2.8%)、101.4%(RSD=1.9%)、101.7%(RSD=1.2%)。结论不同厂家猴头菌片中5种核苷的含有量存在明显差异,应加以关注。AIM To establish an RP-HPLC method for the simultaneous content determination of five nucleosides in Houtoujun Tablets （ myeelia of Hericium erinaceum）. METHODS The analysis of aqueous extract of this drug was performed on a 26 ℃ thermostatic Zorbax SB-Aq C18 column （250 mm ×4.6 mm, 5 μm）, with the mobile phase comprising of methanol-0. 1% phosphoric acid flowing at 0. 5 mL/min in a gradient elution manner, and the detection wavelength was set at 260 nm. RESULTS Cytidine, adenine, uridine, adenosine and guanosine showed good linear relationships within the ranges of 0. 007 - 0. 169, 0. 005 - 0. 115, 0. 032 - 0. 797, 0. 032 - 0. 792 and 0. 004 - 0. 103 ixg, whose average recoveries （ n = 6） were 99.4% （ RSD = 2. 0% ）, 98.3% （ RSD = 1.8%）, 98.0% （RSD=2.8%）, 101.4% （RSD=l.9%） and101.7% （RSD=1.2%）, respectively. CON- CLUSION The contents of five nueleosides in Houtoujun Tablets from different manufacturers exhibit obvious differences, to which we should pay attention.

关 键 词：猴头菌片 胞苷 腺嘌呤 尿苷 腺苷 鸟苷 RP-HPLC 

分 类 号：R927.2[医药卫生—药学]