5个采收期女贞子中5种成分的动态变化  

Dynamic changes of five constituents in Ligustri lucidi Fructus at five picking time

作　　者：王甫成[1] 纪东汉 方成武[1,2] 龚道锋[1] 夏成凯[1] 

机构地区：[1]安徽中医药科学院亳州中医药研究所/亳州职业技术学院,安徽亳州236800 [2]安徽中医药大学,安徽合肥230012

出　　处：《中成药》2017年第5期1011-1014,共4页Chinese Traditional Patent Medicine

基　　金：安徽省高等学校省级自然科学研究重点项目(KJ2016A498);安徽省高等教育振兴计划人才项目[皖教秘人(2014)181];安徽省高等学校质量工程项目(2015msgzs174;2015jxtd073;2015sjjd039;2014jxtd073)

摘　　要：目的分析5个采收期(8、9、10、11、12月)女贞子Ligustri lucidi Fructus中5种成分的动态变化。方法女贞子乙醇提取液的HPLC分析采用Aglient Zorbax SB-C_(18)色谱柱(4.6 mm×250 mm,5μm);以乙腈-0.1%磷酸为流动相,梯度洗脱;体积流量1.0 m L/min;柱温25℃;检测波长224 nm。结果红景天苷、酪醇、木犀草苷、女贞苷和特女贞苷在各自范围内均呈良好的线性关系(r>0.999 0),加样回收率99.56%~100.30%,RSD 0.89%~1.23%。各成分(除酪醇外)含有量均在9月采收的样品中最高,10月次之。结论女贞子的适宜采收期为9月和10月。AIM To analyze the dynamic changes of five constituents in time （ August, September, October, November, December）. METHODS Fructus ethanol extract was performed on a 25 ℃ thermostatic Aglient Zorbax Ligustri lucidi Fructus at five picking The HPLC analysis of Ligustri lucidi SB-Cls column （4. 6 mm ×250 mm, 5 μm）, with the mobile phase comprising of acetonitrile-0. 1% phosphoric acid flowing at 1 mL/min in a gradient elution manner, and the detection wavelength was set at 224 nm. RESULTS Salidroside, tyrosol, luteolin-7-O- glucoside, ligustroflavone and specnuezhenide showed good linear relationships within their own ranges （r 〉 0. 999 0）, whose average recoveries were 99.56% - 100. 30% with the RSDs of 0. 89% - 1.23% . The contents of various constituents （except for tyrosol） were the highest in samples picked up in September, followed by those picked up in October. CONCLUSION The suitable picking time of Ligustri lucidi Fructus is September and October.

关 键 词：女贞子 红景天苷 酪醇 木犀草苷 女贞苷 特女贞苷 采收期 HPLC 

分 类 号：R284.1[医药卫生—中药学]