氧化苦参碱对乙肝病毒的抑制作用及对miR-122表达的影响  

Inhibitory effect of oxymatrine on hepatitis B virus and its effect on expression of mircro RNA-122

作　　者：张轩[1] 党秀敏 肖倩[1] 李有强[1] 刘健平[1] 王淏[2] 

机构地区：[1]广东省中医院,广州中医药大学第二附属医院,广州市510120 [2]广州血液中心,510095

出　　处：《实用医学杂志》2017年第9期1402-1404,共3页The Journal of Practical Medicine

基　　金：广东省中医药局基金资助项目(编号:20152140)

摘　　要：目的:探讨氧化苦参碱抗乙型肝炎病毒的作用机制。方法:用不同浓度的氧化苦参碱处理Hep G2.2.15细胞,采用cck-8法检测不同药物浓度对Hep G2.2.15细胞增殖的影响;氧化苦参碱处理Hep G2.2.15细胞72 h后收集实验组和对照组上清和细胞,检测氧化苦参碱对上清中HBs Ag、HBe Ag和HBV DNA的抑制作用以及对细胞中mi R-122的表达的影响。结果:药物浓度在4 mg/m L及以下的细胞存活率均高于95%;药物处理Hep G2.2.15细胞后72 h对HBs Ag、HBe Ag和HBV DNA的抑制率分别是66.26%、46.53%和65.95%,mi R-122的相对表达量是对照组的3.5倍。结论:氧化苦参碱有可能通过提高细胞中mi R-122的表达从而抑制病毒的复制和抗原的表达。Objective To investigate whether miR-122 is a target of oxymatrine against HBV. Methods HepG2.2.15 cells were incubated with culture medium containing different concentrations of oxymatrine. The cyto- toxicity of oxymatrine was determined by cck-8 assay. The surface antigen of HBV （HBsAg） , antigenof HBV （HBeAg） and HBV DNA in supernatant and intracellular miR-122 were determined in HepG2.2.15 ceils after incu- bation with culture medium containing oxymatrine for 72 h. Results The survival percentage of HepG2.2.15 cells under different concentrations of oxymatrine was higher than 95% when the concentration of oxymatrine was lower than 4 mg/mL. After treatment with oxymatrine for 72 h, the secretion of HBsAg and HBeAg, the level of HBV DNA in the supernatant were reduced. The intracellular miR-122 in oxymatrine experience group was 3.5 times higher than that in the negative control group. Conclusions Oxymatrine might be used to inhibit viral replication and antigen expression by enhancing the expression of miR-122 in HepG2.2.15 cells.

关 键 词：氧化苦参碱 乙型肝炎病毒 微小核糖核酸122 

分 类 号：R512.62[医药卫生—内科学]