亚胺培南耐药铜绿假单胞菌Ⅰ类整合子检测  

Antibiotic susceptibility and class Ⅰ integron analysis of imipenem-resistant Pseudomonas aeruginosa

作　　者：唐慧琴[1] 陈定强[1] 吴爱武[1] 

机构地区：[1]广州医科大学附属第一医院检验科,510120

出　　处：《实用医学杂志》2017年第9期1494-1497,共4页The Journal of Practical Medicine

摘　　要：目的:了解本院临床分离的亚胺培南耐药铜绿假单胞菌(IRPA)I类整合子基因携带情况,分析其携带的基因盒与细菌耐药的相关性。方法:采用聚合酶链反应(PCR)检测86株IRPA的I类整合酶及I类整合子,测序确定整合子携带的耐药基因盒,采用PCR检测携带整合子耐药基因盒中的金属酶耐药基因、氨基糖苷类修饰酶(AMEs)基因、16Sr RNA甲基化酶基因和Opr D2基因;采用VITEK-2全自动微生物鉴定系统检测整合子阳性菌株的耐药特性。结果:在86株IRPA中,整合酶的检出率为23.3%(20/86),I类整合子的检出率为8.14%(7/86),共检出5种I类整合子耐药基因盒;I类整合子阳性菌株对12种抗菌药物存在不同程度耐药并携带数量众多的耐药基因。结论:I类整合子中携带的多种耐药基因盒与IRPA的多重耐药有关。Objective To investigate the class I integrons and their gene cassettes of imipenem-resistant Pseudomonas aeruginosa （IRPA） , and to analyze the correlation between integrons and drug resistance. Methods PCR was used to determine the presence of integrase genes and class I integrons. The variable regions were detected by sequencing. Resistance genes of integron gene cassettes including metal- β-1actamases, aminoglycoside modifying enzymes （AMEs）, 16SrRNA methylating enzyme and the OprD2 genes were detected by PCR. The VITEK- 2 automated system was used to determine the antibiotic susceptibility of integron-positive IRPA strains. Results The positive rates of integrase genes and class Ⅰintegrons were 23.3% （20/86）and 8.14% （7/86） , and five kinds of gene cassettes were detected in 86 IRPA strains. The class Ⅰ integrons-positive bacterial strains exhibited different resistant patterns to 12 antibiotics with large number of resistance genes. Conclusion The class Ⅰintegrons and their gene cassettes are associated with multiple drug resistance of IRPA.

关 键 词：铜绿假单胞菌 Ⅰ类整合子 耐药基因盒 

分 类 号：R446.5[医药卫生—诊断学]