极地真菌Geomyces sp.3-1次级代谢产物的研究  

Secondary Metabolites of Polar-Derived Fungus Geomyces sp. 3-1

作　　者：李云海[1,2] 王晓丽[1,2,3] 卢小玲[1,2] 于豪冰[1,2] 刘小宇[1,2] 

机构地区：[1]第二军医大学海洋生物医药研究中心 [2]第二军医大学基础医学部生物化学与分子生物学教研室,上海200433 [3]浙江海洋大学海洋科学与技术学院,舟山316022

出　　处：《天然产物研究与开发》2017年第4期590-594,共5页Natural Product Research and Development

基　　金：广东省海洋局公益项目(GD2012-D01-001);国家自然科学基金(41306197;41606173)

摘　　要：采用硅胶柱层析、ODS反相硅胶柱层析、凝胶柱层析和高效液相HPLC等色谱技术,对极地真菌Geomyces sp.3-1的发酵液提取物进行分离纯化,共得到8个化合物,通过波谱解析结合理化性质并比较相关文献,确定化合物为:paulownin(1)、demethylincisterol A3(2)、ergosta-7,22-dienen-3,6-dione(3)、citreoanthrasteroid B(4)、19-norergosta-5,7,9,22-tetraene-3β-ol(5)、(22E)-5α,8α-epidioxyergosta-6,22-dien-3β-ol(6)、(3β,5α,6β,22E)-6-methoxyergosta-7,22-diene-3,5-diol(7)和ergosta-7,22-dien-3β-ol(8)。其中,化合物2~7均是首次从Geomyces属真菌中获得,化合物2有较好的抗菌和细胞毒活性。Eight compounds were obtained from the fermentation of the polar-derived fungus Geomyces sp. 3-1. The ex- tract was isolated and purified by column chromatography on silica-gel, reversed phase chromatography, Sephadex LH-20 and HPLC. The structures of compounds were elucidated by physical and chemical properties and spectral analysis, and comparison with the data of literatures. The eight compounds were identified as panlownin （1）, demethylincisterol A3 （ 2）, ergosta-7,22-dienen-3,6-dione （ 3 ）, citreoanthrasteroid B （4）, 19-norergosta-5,7,9,22-tetraene-3β-ol （ 5 ）, （22E） - 5α,8α-epidioxyergosta-6,22-dien-3β-ol（6） ,（3β,5α,6β,22E）-6-methoxyergosta-7,22-diene-3,5-diol（7） and ergosta- 7,22-dien-3β-ol（8）. Compounds 2-7 were firstly isolated from Geomycesspecies,and compound 2 was shown to have an- tibacterial and eytotoxic activities.

关 键 词：极地真菌 地丝霉属 次级代谢产物 生物学活性 

分 类 号：R9[医药卫生—药学]