光敏功能化载药系统CPTPP-FA-LCOS的制备及性能  

Preparation and Properties of Drug Delivery System CPTPP-FA-LCOS with Photosensitive Function

作　　者：黄燕[1] 朱卫华[1] 华明清 张岐[1,2] 张小燕[1] 吴静波[1] 

机构地区：[1]江苏大学化学化工学院,江苏镇江212013 [2]海南大学海南省精细化工重点实验室,海南海口570228

出　　处：《精细化工》2017年第5期494-498,共5页Fine Chemicals

基　　金：国家自然科学基金(21601068/21471069/21302071);江苏省高等自然科学研究面上项目(14KJB150006);江苏省博士后科研资助计划项目(1401050C);江苏大学高级人才科研启动基金(14JDG054)~~

摘　　要：通过酰化反应将叶酸(FA)、两亲性亚油酰化壳寡糖(LCOS)连接到羧基取代的四苯基卟啉(TPP)化合物5-(4-羧基苯基)-10,15,20-三苯基卟啉(CPTPP)结构上,得到了潜在光敏功能化载药系统CPTPP-FA-LCOS。利用FTIR、UV-Vis、TEM、FL对CPTPP-FA-LCOS的结构进行了表征,并运用表面张力法测定了其临界胶束质量浓度(CMC)。结果表明:CPTPP-FA-LCOS的CMC=2.5×10^(-3)g/L,其可形成稳定的球形纳米胶束,粒径在70~120 nm。四唑盐(MTT)比色法的初步实验结果表明:在光照条件下,0.4 g/L的CPTPP-FA-LCOS即可使He La细胞存活率降至50%以下,表明CPTPP-FA-LCOS具有明显的光毒性。A photosensitive drug delivery system CFFPP-FA-LCOS was obtained by acylation reaction of folic acid （FA）, amphiphilic N-linoleyl chitosan oligosaccharide （LCOS） with carboxyl substituted meso-tetraphenylporphyrin （TPP） of 5- （4-carboxyl） phenyl-10,15,20-triphenyl porphyrin （ CPTPP ）. The chemical structure of CPTPP-FA-LCOS was characterized by FTIR, UV-Vis,TEM, and fluorescence spectrum. The critical micefle concentration （CMC） of CFI＇PP-FA-LCOS was also determined using surface tension method. The results showed that CMC value of CPTPP-FA-LCOS was 2.5× 10-3 g/L. CPTPP-FA-LCOS could form stable spherical nano-micelles with a particle size between 70 and 120 nm. The results of preliminary MTI＂ （methyl thiazolyl tetrazolim ） suggested that 0. 4 g/L CPTPP-FA-LCOS could make the HeLa cell viability reduce to less than 50% under the light condition, indicating that CPTPP-FA-LCOS had obvious phototoxicity.

关 键 词：光动力疗法 光敏剂 亚油酰化壳寡糖 叶酸 功能材料 

分 类 号：O644[理学—物理化学]