iTRAQ多重化学标记串联质谱技术在生命科学领域中的应用  

iTRAQ Multiple Chemical Markers Tandem Mass Spectrometry Technology in Life Sciences

作　　者：白龙[1] 乌日罕[1] 孟永梅[1] 

机构地区：[1]内蒙古医科大学,内蒙古呼和浩特010110

出　　处：《中华中医药学刊》2017年第5期1155-1157,共3页Chinese Archives of Traditional Chinese Medicine

基　　金：国家自然科学基金项目(81260596);国家中医药管理局中医药行业科研专项(200807007)

摘　　要：同位素标记相对和绝对定量(isobaric tags for relative and absolute quantitation,iTRAQ)技术—利用多种同位素试剂标记蛋白多肽N末端或赖氨酸侧链基团,经高精度质谱仪串联分析,可同时比较多达8种样品之间的蛋白表达量,是近年来定量蛋白质组学常用的高通量筛选技术,正迅速被广大学者所接受。主要从iTRAQ实验技术概要、iTRAQ多重化学标记串联质谱技术在生命科学领域中的应用、问题与展望等三个方面进行简要总结。Isotope labeling relative and absolute quantitation （isobaric tags for relative and absolute quantitation, iTRAQ） technology - the use of a variety of isotopically labeled polypeptide reagent N - terminus or lysine side chain group, the high- precision mass spectrometer series analysis, can compare up to the amount of protein expression be- tween samples of eight kinds and is a high - throughput screening technology commonly used for quantitative proteomics in recent years. It is rapidly being accepted by the majority of scholars. The following describes the iTRAQ experimental techniques, iTRAQ three aspects of multiple chemical markers tandem mass spectrometry in the field of life science appli- cations, problems and prospects and other brief summary.

关 键 词：相对和绝对定量同位素标记(iTRAQ)技术 生命科学 

分 类 号：R2-03[医药卫生—中医学]