温经化瘀止痛法对寒凝血瘀证原发性痛经大鼠血清E_2、P、PGE_2、PGF_(2α)、TXB_2及6-Keto-PGF_(1α)的影响  

Effect of Wenjing Huayu Zhitong Treatment on E_2,P,PGE_2,PGF_(2α),TXB_2 and 6-Keto-PGF_(1α) in Rats with Primary Dysmenorrhea of Syndrome of Blood Stasis Due to Cold Accumulation

作　　者：马青[1] 师伟[2] 刘少玲 李霞[4] 赖楠楠 王东梅[2] 

机构地区：[1]山东中医药大学第一临床医学院,山东济南250355 [2]山东中医药大学附属医院,山东济南250014 [3]烟台市中医医院,山东烟台264000 [4]山东省医学科学院基础医学研究所,山东济南250062

出　　处：《中华中医药学刊》2017年第5期1281-1284,共4页Chinese Archives of Traditional Chinese Medicine

基　　金：山东省重点研发计划项目(2015GSF119010)

摘　　要：目的:通过研究温经化瘀止痛法对寒凝血瘀证原发性痛经大鼠血清雌二醇(E_2)、孕酮(P)、前列腺素E_2(PGE_2)、前列腺素F_(2α)(PGF_(2α))、血栓素B2(TXB2)及6-酮-前列腺素F_(1α)(6-Keto-PGF_(1α))的影响,探讨其治疗痛经的作用机制。方法:参考文献法建立寒凝血瘀证原发性痛经大鼠模型,分为空白组、模型组、中药小剂量组、中药大剂量组和西药组,分别给药6 d,于末次给药30 min后,除空白组外,其余各组大鼠分别腹腔注射缩宫素,观察其30 min内扭体反应次数,并检测各组大鼠血清E_2、P、PGE_2、PGF_(2α)、TXB2及6-Keto-PGF_(1α)的含量。结果:中药小剂量组和中药大剂量组均可延长寒凝血瘀证原发性痛经大鼠疼痛潜伏时间,减少其子宫痉挛收缩引起的疼痛反应,降低血清E_2、PGF_(2α)和TXB2的含量,有效升高血清PGE_2及6-Keto-PGF_(1α)的水平,与模型组相比较,差异有统计学意义(P<0.05);中药大剂量组血清P含量较模型组增加,但差异无统计学意义(P>0.05)。结论:温经化瘀止痛法的止痛作用机制,可能与其调控寒凝血瘀证原发性痛经大鼠血清前列腺素、E_2含量以及TXB2与6-Keto-PGF_(1α)比值有关。Objective： To investigate the effect and mechanism of Wenjing Huayu Zhitong treatment on estradiol,pro- gesterone,prostaglandin E2 ,prostaglandin F2, ,Thromboxane B2 and 6- keto- prostaglandin FI, in rats with primary dys- menorrhea of syndrome of blood stasis due to cold accumulation. Methods ： The rat model of primary dysmenorrhea of syn- drome of blood stasis due to cold accumulation was induced by oxytocin and cold. All the 50 rats were randomly divided into normal group, model group, TCM low and high dose groups and ibuprofen group. Each group was given drugs for six consecutive days except the normal group and injected oxytocin 30 minutes after last administration, for the purpose to ob- serve the frequency of writhing response in 30 minutes and detect the levels of E2 , P, PGE2 , PGF2α, TXB2 and 6 - Keto -PGF1α in rats serum. Results： Compared with model group, the latency period of writhing response was extended and the frequency of the writhing response was less in TCM low high dose groups, while the contents of E2 , PGF1α and TXB2 decreased and the contents of PGE2 and 6 - Keto - PGF1 α increased with significant difference （ P 〈 0. 05 ）. The contents of P increased in TCM high dose group compared with that of the model group without significant difference （ P 〉 0. 05 ）. Conclusion： The mechanism of Wenjing Huayu Zhitong treatment on primary dysmenorrhea might be concerned with the modulation role to the contents of prostaglandin and estrogen and the ratios of TXB2/6 - Keto - PGF1α.

关 键 词：温经化瘀止痛法 香延止痛方 原发性痛经 寒凝血瘀证 前列腺素 雌、孕激素 血栓素B2 6-酮-前列腺素F1Α 

分 类 号：R271.113[医药卫生—中医妇科学]