土壤盐分胁迫对甜高粱茎秆糖分积累及蔗糖代谢相关酶活性的影响  

Effect of salt soil stress on sugar accumulation and sucrose-metabolizing enzymes of sweet sorghum stem

作　　者：刘海波[1] 魏玉清[1] 周维松[1] 邹程[1] 

机构地区：[1]北方民族大学生物科学与工程学院,宁夏银川750021

出　　处：《西北农林科技大学学报（自然科学版）》2017年第5期41-47,56,共8页Journal of Northwest A&F University(Natural Science Edition)

基　　金：国家自然科学基金项目(31060180);北方民族大学重点科研项目(2015KJ38)

摘　　要：【目的】研究土壤盐分胁迫对甜高粱茎秆中糖分积累及蔗糖代谢相关酶活性的影响,探明盐胁迫条件下甜高粱茎秆的糖分积累特征。【方法】以甜高粱杂交品种"辽甜1号"为研究材料,采用高效液相色谱法和酶学测定方法,对5种不同土壤盐分(分别为对照CK(0.102%)、T1(0.215%)、T2(0.250%)、T3(0.300%)、T4(0.459%),以上为质量分数)胁迫下,甜高粱各生育时期(拔节期、孕穗期、开花期、灌浆期、成熟期)茎秆中的糖分(葡萄糖、果糖、蔗糖)含量和糖代谢相关酶(蔗糖磷酸合成酶(SPS)、蔗糖合成酶合成方向(SS-s)、蔗糖合成酶分解方向(SS-d)、中性转换酶(NI)、酸性转换酶(AI))活性进行测定。【结果】(1)甜高粱茎秆总糖在灌浆期积累最多,蔗糖是影响总糖含量的主要因素,土壤盐质量分数在0.215%~0.300%时,总糖、蔗糖含量随盐胁迫程度加深呈现增加的趋势。(2)低盐胁迫(0.215%~0.300%)可使甜高粱茎秆中SPS、SS-d、SS-s、AI活性增加,而对NI活性无显著影响。(3)甜高粱茎秆中总糖含量与蔗糖含量和AI活性呈显著正相关,而与其他酶无显著相关性。【结论】甜高粱茎秆糖分累积是各种酶共同作用的结果,其中AI是调控甜高粱茎秆总糖的关键酶。[Objective] The effect of soil salt stress on sugar content and activities of sucrose metabo- lism related enzymes of sweet sorghum stalks were studied. [Method] The sweet sorghum hybrid Liao- tian-1 was tested under five different salinity soils （including control CK 0. 102%, T1 0. 215%, T2 0. 250% ,T3 0. 300% and T4 0. 459% in mass fraction）. The contents of stalk glucose,fructose and sucrose and activities of sugar metabolism related enzymes such as sucrose phosphate synthase, sucrose synthase synthesis direction （SS-s）, sucrose synthase decomposition direction （SS-d）, neutral converting enzyme （NI） and acid converting enzyme （AI） at five different growth periods （jointing stage and booting stage, flowering period,filling stage,and mature period） were determined by HPLC and enzyme method. IRe- sult] 1） Sweet sorghum stalks total sugar accumulation in the mature period was the highest among differ- ent periods. Sucrose was the dominant factor influencing the total sugar content, and the total sugar and sucrose content of stalk was higher when soil salt concentration mass fraction was 0. 215%-0. 300% com-pared to other salt stresses. 2） Low soil salt stress （0. 215%-0. 300%） increased activities of SPS,SS-d, SS-s and AI,but had no significant effect on NI. 3） The total sugar were significantly correlated with su- crose and AI with correlation coefficients of 0. 935 and -0. 892. [Conclusion] Sweet sorghum stalks sugar accumulation was controlled by various enzymes and AI was the key regulation enzyme for total sugar of sweet sorghum stalks.

关 键 词：土壤盐分 甜高粱 糖分积累 糖代谢相关酶 

分 类 号：S514.01[农业科学—作物学]