高原低氧加重脂多糖诱导大鼠肺组织的慢性炎症  

High altitude hypoxia aggravates chronic inflammation induced by LPS in rats

作　　者：张益[1] 李光宗[2] 郁硕 陈锋[2] 刘英富[2] 霍景瑞[2] 郑淑芳[3] 

机构地区：[1]锦州医科大学武警后勤学院附属医院研究生培养基地,天津300162 [2]武警后勤学院附属医院救援医学研究所,天津300162 [3]武警后勤学院附属医院病理科,天津300162

出　　处：《军事医学》2017年第4期273-277,共5页Military Medical Sciences

基　　金：天津市科技计划资助项目(14ZCDZSY00033);武警后勤学院附属医院种子基金资助项目(FYM201514);武警后勤学院附属医院科研平台开放基金资助项目(WYKFZ201603;WYKFM201602);全军重点实验室开放基金资助项目(JY1402)

摘　　要：目的探讨高原低氧对大鼠慢性炎症的影响。方法 40只SD大鼠随机均分为正常对照组(对照组)、慢性炎症组(慢炎组)、高原低氧组(高低组)、高原低氧+慢性炎症组(高+慢组);慢炎组大鼠尾静脉注射脂多糖(LPS,0.5 mg/kg),每周2次,连续注射4周,高+慢组经慢炎组相同处理后,同高低组大鼠一起置于模拟海拔6000m的高原低氧环境中连续3 d;然后进行肺组织取材、病理切片HE染色观察。外周血白细胞分类和计数,酶联免疫吸附实验(ELISA)分别检测血清和肺组织IL-6、TNF-α水平,Western印迹检测肺组织IL-6蛋白表达。结果经LPS和高原低氧暴露处理过的大鼠肺组织出现炎细胞浸润、肺泡间毛细血管扩张的病理学变化;外周血白细胞分类和计数。ELISA结果显示,与对照组相比,慢炎组、高低组均能导致大鼠外周血白细胞总数增加(P<0.01),血清和肺组织IL-6和TNF-α水平均升高(均P<0.05);高原低氧和慢性炎症具有交互作用(P<0.01),高+慢组进一步增加了外周血白细胞数,并促进IL-6和TNF-α的表达升高(P<0.01)。结论通过大鼠脂多糖慢性炎症模型,证实高原低氧可加重慢性炎症发展。Objective To investigate the effect of high altitude hypoxia on chronic inflammation in rats.Methods Forty SD rats were randomly divided into 4 groups：control group（Con）,chronic inflammation group（CI）,high altitude hypoxia group（HH）,high altitude hypoxia ＋ chronic inflammation group（HH ＋ CI）.Rats in CI group were injected with lipopolysaccharide（LPS）（0.5 mg/kg） through the caudal vein twice a week for 4 weeks.Rats in HH ＋ CI group were treated just as CI group was,but together with HH group rats were settled in a hypoxic environment of 6000 m altitude for three days.Pathological changes in lung tissues were observed by hematoxylin eosin stain.The peripheral white blood cell count and classification were measured.The levels of interleukin-6（IL-6） and tumor necrosis factor alpha（TNF-α） in serum and lung tissues were detected by enzyme-linked immunosorbent assay（ELISA）.Changes in IL-6 expression in rat lung tissues were observed by Western blotting.Results After LPS and high altitude hypoxia exposure,inflammatory cells infiltration and alveolar capillary expansion were observed in rats＇ lung tissue.Compared with Con group,not only the peripheral white blood cell count,but also the level of IL-6 and TNF-α in serum and lung tissue increased in CI and HH group（P〈0.01）.IL-6 expression levels observed by Western blotting were also increased in HH and CI group（P〈0.01）.High altitude hypoxia and chronic inflammation interacted（P〈0.01）.The peripheral white blood cell count was higher in HH ＋ CI group than in other groups,and IL-6 and TNF-α expressions in lung tissue were increased（P〈0.05）.Conclusion An LPS-induced chronic inflammation model in rats is successfully obtained,and high altitude hypoxia could aggravate chronic inflammation.

关 键 词：炎症 高原低氧 脂多糖类 白细胞介素6 肿瘤坏死因子α 

分 类 号：R594.3[医药卫生—内科学]