天津某小区生活饮用水中胞外耐药基因污染分析  

Extracellular antibiotic resistance genes in tap water in a district of Tianjin

作　　者：刘珊珊[1,2] 侯爱明 张坤明[2] 杨栋[2] 刘伟丽[2] 邱志刚[2] 谌志强[2] 曲红梅[1] 李君文[2] 金敏[2] 

机构地区：[1]天津大学化工学院,天津300350 [2]军事医学科学院卫生学环境医学研究所,天津市环境与食品安全风险监控技术重点实验室,天津300050

出　　处：《军事医学》2017年第4期282-286,共5页Military Medical Sciences

基　　金：国家自然科学基金面上项目(81372947)

摘　　要：目的研究天津某小区生活饮用水中胞外耐药基因的污染特征,阐明生活饮用水中胞外耐药基因污染规律。方法于2015年12月至2016年11月,利用自行研制的核酸吸附柱富集生活饮用水中的游离核酸,采用实时荧光定量PCR技术检测tetC、ermB、ampC、sul1、qnrA、aadA、catA1和katG 8种胞外耐药基因的浓度。结果与结论在12份水样中,tet C的检出率最高,约为66.7%;其次为sul1和qnr A(41.7%)、ampC(33.3%)、aadA(16.7%)、cat A1和kat G(均为8.33%),erm B未检测出。生活饮用水中以sul1胞外耐药基因的污染最严重,达(1.27±0.04)×10~5GC/L。各胞外耐药基因出现最大浓度的时间主要集中在6月,且3月和6月检测出的胞外耐药基因种类最多,达5种。天津某小区生活饮用水中存在多种胞外耐药基因的污染。Objective To study the pollution characteristics of extracellular antibiotic resistance genes（e ARGs） in tap water in a district in Tianjin and to find the patterns of change of eARGs.Methods Tap water samples were collected and enriched by nucleic acid adsorption particle column between December 2015 November 2016.Eight eARGs,including tetC,ermB,ampC,sul1,qnrA,aad A,catA1 and katG,were detected by qPCR.Results and Conclusion In the twelve water samples,tet C showed the highest detection rate of 66.7%,followed by sul1 and qnr A（41.7%）,amp C（33.3%）,aad A（16.7%）,cat A1 and kat G（8.33%）.erm B was not detected.Sul1 showed the highest concentration of（1.27±0.04）×10^5GC/L.In June,most of the e ARGs reached the highest concentration.In March and June,as many as five types of resistant genes were detected.There are many extracellular resistance genes in tap water.

关 键 词：生活饮用水 耐药基因 胞外耐药基因 

分 类 号：R123[医药卫生—环境卫生学]