低氧因素对丙泊酚麻醉新生大鼠海马长时程增强的影响  

Effect of hypoxemia factor on hippocampal long-term potentiation in newborn rats undergoing propofol anesthesia

作　　者：涂生芬[1] 陈恒胜 孙茫[1] 袁心刚 杨飞[1] 吴胜德 魏光辉 

机构地区：[1]重庆医科大学附属儿童医院麻醉科,400014 [2]儿童学重庆市重点实验室 [3]儿童学重庆市重点实验室泌尿外科 [4]儿童发育重大疾病国家国际科技合作基地

出　　处：《中华麻醉学杂志》2017年第3期275-278,共4页Chinese Journal of Anesthesiology

基　　金：国家自然科学基金（31200853）;重庆市自然科学基金（cstc2012jjA10036）;重庆市卫计委高端后备人才项目（2015HBRC007）;国家临床重点专科建设项目（国卫办医函[2013]544）

摘　　要：目的评价低氧因素对丙泊酚麻醉新生大鼠海马长时程增强（LTP）的影响。方法清洁级健康新生sD大鼠42只，7日龄，体重14～18g，雌雄各半。采用随机数字表法分为3组（n=14）：丙泊酚＋空气组（PA组）和丙泊酚＋纯氧组（PO组）分别腹腔注射丙泊酚50mg／kg，1次／d，连续7d；脂肪乳＋纯氧组（10组）腹腔注射脂肪乳5．0ml／kg，1次／d，连续7d。每次注射完毕后分别置于空气和纯氧环境中6h。给药后测定动脉血氧饱和度（SaO2）和呼吸频率（RR），待翻正反射恢复后放回鼠笼。各组随机取6只大鼠于第7天注射完毕后24h，制备海马脑片，记录电刺激诱导的兴奋性突触后场电位（fEPSP）和LTP诱导成功率。余大鼠给药后2周进行Morris水迷宫实验，测定认知功能。结果与10组比较，PO组RR、fEPSPS幅度和LTP诱导成功率降低，逃避潜伏期延长（P〈0．05）；与PO组比较，PA组SaO2、fEPSPS幅度和LTP诱导成功率降低，逃避潜伏期延长，穿越原平台区次数减少（P〈0．05）。结论低氧因素可增强丙泊酚麻醉新生大鼠的中枢神经毒性。Objective To evaluate the effect of hypoxemia factor on hippocampal long-term potenti- ation （LTP） in newborn rats undergoing propofol anesthesia. Methods Forty-two pathogen-free healthy Sprague-Dawley rats （21 males, 21 females）, aged 7 days, weighing 14-18 g, were divided into 3 groups （n= 14 each） using a random number table： propofol plus air group （group PA） , propofol plus pure oxygen group （group PO） and intralipid plus pure oxygen group （group IO）. Propofol 50 mg/kg was injected intraperitoneally once a day for 7 consecutive days in PA and PO groups. Intralipid 5.0 ml/kg was injected intraperitoneally once a day for 7 consecutive days in IO group. The rats were exposed to air or pure oxygen for 6 h after the end of each injection. The arterial oxygen saturation and respiratory rate were deter- mined after administration. The rats were returned to the cage after recovery of righting reflex. Six rats in each group were selected for preparation of hippocampal slices at 24 h after the last injection on 7th day,and the electric stimulation-induced field excitatory post synaptic potential （fEPSP） and success rate of LTP induction were recorded. Morris water maze test was performed in the other rats at 2 weeks after admin- istration to assess the cognitive function. Results Compared with group IO, the respiratory rate, ampli- tude of fEPSP and success rate of LTP induction were significantly decreased, and the escape latency was prolonged in group PO （P〈0. 05）. Compared with group PO, the arterial oxygen saturation, amplitude of fEPSP and success rate of LTP induction were significantly decreased, the escape latency was prolonged, and the number of crossing the original platform was decreased in group PA （P〈0.05）. Conclusion Hypoxemia factor increases propofol-induced neurotoxicity in the central nervous system of newborn rats.

关 键 词：氧 二异丙酚 海马 长时程增强 认知 

分 类 号：R722.1[医药卫生—儿科]