雷公藤红素对小鼠盐酸吸入性急性肺损伤的影响  

Effect of tripterine on hydrochloric acid-induced acute lung injury in mice

作　　者：王君涛[1] 刘娟 褚海辰[1] 张晓[1] 袁莉[1] 董河[1] 

机构地区：[1]青岛大学附属医院麻醉科,266000 [2]青岛市海慈医疗集团,266000

出　　处：《中华麻醉学杂志》2017年第3期382-384,共3页Chinese Journal of Anesthesiology

摘　　要：目的评价雷公藤红素对小鼠盐酸吸入性急性肺损伤的影响。方法清洁级健康成年雄性ICR小鼠18只，7～9周龄，体重25～30g，采用随机数字表法分为3组（n=6）：对照组（C组）、盐酸吸入性急性肺损伤组（ALI组）和雷公藤红素组（T组）。采用气管内滴注盐酸的方法制备小鼠吸入性急性肺损伤模型。T组腹腔注射雷公藤红素3mg／kg，1次／d，连续3d，随后制备模型。于滴注后6h时处死小鼠取肺，观察肺组织病理学结果，测定TNF—α、IL-6、巨噬细胞移动抑制因子（MIF）和髓过氧化物酶（MPO）的水平。结果与C组比较，ALI组和T组滴注后6h时肺组织TNF—d、IL-6、MIF和MPO的水平升高（P〈0．01）；与ALI组比较，T组滴注后6h时肺组织TNF—d、IL-6、MIF和MPO的水平降低（P〈0．01）。T组肺组织病理学损伤较ALI组减轻。结论雷公藤红素可减轻小鼠盐酸吸入性急性肺损伤。Objective To evaluate the effect of tripterine on hydrochloric acid-induced acute lung injury （ALl） in mice. Methods Eighteen pathogen-free healthy adult male ICR mice, aged 7-9 weeks, weighing 25-30 g, were divided into 3 groups （n=6 each） using a random number table： control group （group C）, hydrochloric acid-induced ALI group （group ALI） and tripterine group （group T）. ALI was induced by a single intratracheal instillation of hydrochloric acid 2 ml/kg （pH 1.5） via a 24-gauge anglo- catheter inserted into the trachea in pentobarbital sodium-anesthetized mice. Tripterine 3 mg/kg was injected intraperitoneally once a day for 3 consecutive days, and then the model was established in group T. The mice were sacrificed at 6 h after instillation, and lung specimens were obtained for microscopic examination and for determination of the levels of tumor necrosis factor-alpha （TNF-c~）, interleukin-6 （ IL-6）, macro- phage migration inhibitory factor （MIF） and myeloperoxidase （MPO） in lung tissues. Results Com- pared with group C, the levels of TNF-c~, IL-6, MIF and MPO were significantly increased at 6 h after in- stillation in ALI and T groups （P〈0.01）. Compared with group ALI, the levels of TNF-α, IL-6, MIF and MPO were significantly decreased at 6 h after instillation in group T （P〈0.01）. The pathological chan- ges of lung tissues were significantly attenuated in group T compared with group ALI. Conclusion Tripter- ine can attenuate hydrochloric acid-induced ALI in mice.

关 键 词：雷公藤属 盐酸 急性呼吸窘迫综合征 成人 

分 类 号：R614[医药卫生—麻醉学]