不同小干松种源的SRAP分析  

SRAP Analysis of 15 Provenances of Pinus contorta

作　　者：王骞春[1] 

机构地区：[1]辽宁省林业科学研究院,辽宁沈阳110032

出　　处：《安徽农业科学》2017年第12期139-140,156,共3页Journal of Anhui Agricultural Sciences

基　　金：国家林业局"948"项目"北美小干松优良遗传资源及高效育种技术引进"

摘　　要：[目的]研究小干松种源遗传多样性,为小干松引种、育种工作提供基础资料。[方法]利用SRAP荧光标记技术对引种的15个小干松种源进行SRAP分析。[结果]9对荧光标记的SRAP引物共产生1 075个条带,多态性位点953个,平均多态性为88.7%,每对引物扩增条带75~146条,平均每对引物扩增条带119条;通过UPGMA软件对15个种源进行遗传聚类分析,15个种源的相似系数在0.761 3~0.876 0,以遗传相似系数0.850 0为阈值可将15个种源分为4个群体,同一地理来源的种源有一定的遗传差异,但大体呈现按地理来源聚类的趋势。[结论]15个小干松种源具有较高的基因多态性,遗传多样性丰富,小干松变异类型与地理分布有关。[ Objective ] To study the gentic diversity of Pinus contorta provenance for laying the foundation for＂introduction of P. contorta breeding. [ Method] Using SRAP fluorescence technique to analyze 15 provenances of P. contorta. [ Result]9 SRAP primers for fluorescent labeling produced a total of 1 075 bands, 953 polymorphic loci, the average polymorphism was 88.7%. Each primer amplified bands were from 75 to 146, an average of 119 bands. The similarity coefficients of 15 provenances were 0.7613 -0. 876 0 by UPGMA software. Taking the genetic similarity coefficient of 0. 850 0 as threshold, 15 provenances were divided into 4 clusters, the provenances with the same geo- graphic origin had some genetic differences, but generally showed a trend of clustering according to geographic origin. [ Conclusion] 15 prove- nances of P. contorta had high genetic diversity, abundant genetic diversity, the variation types were related to geographic distribution.

关 键 词：小干松 种源 SRAP 

分 类 号：S566.301[农业科学—作物学]