Anti-stress Effects of Tunisian Cymbopogon schoenanthus L. Ethanol Extract and Some of Its Active Compounds  

作　　者：Mahmoud Ben Othman Mohamed Neffati Hiroko Isoda 

机构地区：[1]Alliance for Research on North Africa (ARENA), University of Tsukuba, 1-1-1 Tennodai, Tsukuba City, Ibaraki 305-85 72, Japan [2]Faculty of Life and Environmental Sciences, University of Tsukuba, 1-1-1 Tennodai, Tsukuba City, lbaraki 305-8572, Japan [3]Range Ecology Laboratory, Arid Land Institute of Medenine, Medenine 4119, Tunisia

出　　处：《Journal of Agricultural Science and Technology(A)》2017年第2期91-99,共9页农业科学与技术（A）

摘　　要：Many antioxidant rich phytochemical extracts are attracting interest as stress adaptogens. In this regard, several plants are used traditionally and clinically for the management of neurologic disorders. Here, the anti-stress properties of the ethanol extract of medicinal plant Cymbopogon schoenanthus （CSEE）, which grows wild in Southern Tunisia, were investigated, as well as the effects of some of the extracted active compounds on H202-induced cytotoxicity, overproduction of reactive oxygen species （ROS） and adenosine triphosphate （ATP） depletion in human neuroblastoma SH-SYSY cells. Whereas fluorescence intensity due to DCFH-DA （a marker of ROS production） in H2O2-treated SH-SY5Y cells was greater than that in untreated control cells, pretreatrnent with CSEE and its active compounds ameliorated H2O2-induced ROS production. Moreover, H2O2 at 150 μM significantly decreased intracellular ATP levels in SH-SY5Y cells, and pretreatment with CSEE and its active compounds buffered this effect. These in vitro results demonstrate the potential of CSEE to protect against stress-associated disorders.

关 键 词：ATP levels H2O2 medicinal plant reactive oxygen species stress. 

分 类 号：S[农业科学]