小鼠肝脏冷缺血状态下细胞凋亡及长链非编码RNA表达情况的试验研究  

Experimental study on apoptosis and expression of long non-coding RNA in mouse liver under cold ischemia

作　　者：苏松 彭晓智[2] 刘江 罗德 刘向东 李波 

机构地区：[1]西南医科大学附属医院肝胆外科,四川泸州646000 [2]海南省农垦三亚医院内分泌科

出　　处：《西南医科大学学报》2017年第2期109-112,共4页Journal of Southwest Medical University

基　　金：四川省人事及社会保障厅归国留学人才择优资助项目(2010-1021);四川省科技厅科研资助项目(2014JY0068)

摘　　要：目的:探讨小鼠肝脏冷缺血模型肝脏组织细胞凋亡及长链非编码RNA(lnc RNA)的表达情况。方法:C57BL/6小鼠随机分为假手术组(Sham组)及冷保存组(CS组),CS组将小鼠肝脏切除后置于4℃的UW液内保存24 h。Sham组仅做肝脏游离后关腹,24 h后切除肝脏。HE染色观察肝脏组织病理变化,TUNEL法检测细胞凋亡水平,RNA测序法评两组lnc RNA表达谱的变化,并通过实时定量PCR验证测序法筛选出的lnc RNA。结果:与Sham组相比,CS组的病理损伤评分明显增加,细胞凋亡水平明显上升(P<0.05)。与Sham组相比,CS组中WDR26-3、RNF34、FEZ1-2、TUG1及SPRED2-1的lnc RNA明显下调,SERl2-3、DLX2-2、MERRC10、ZFHx3-1和CNOT2的lnc RNA明显上调。结论:在肝脏冷缺血状态下,存在明显细胞凋亡现象以及lnc RNA表达水平改变,探索两者的调控关系可能为减轻肝脏冷缺血损伤找到新的靶点。Objective： To investigate apoptosis and the expression of long non-coding RNA in liver during cold storage in a mouse cold ischemia model. Methods：The C57BL/6 mice were randomly divided into two groups, sham group and cold storage group （CS group）. In CS group, livers were removed and stored in UW solution for 24 h at 4°C. In sham group, mice were undergone laparotomy and livers were cut free from all ligaments. The abdomens were closed and livers were harvested after 24 h. The histological changes and apoptosis of hepatocyte were evaluated by HE staining and TUNEL respectively. The RNAs were extracted and sequenced. The results were analyzed by bioinformatics. Results： Compared with sham group, the histological damage and apotopsis of hepatocytes were significantly promoted in CS group （P 〈 0.05）. Meanwhile, in CS group lncRNA-WDR26-3, RNF34, FEZ1-2, TUG1, SPRED2-1 were down-regulated and SERl2-3,DLX2-2,MERRC10, ZFHx3-1,CNOT2 were up-regulated. Conclusion： Cold ischemia significantly changed the apoptosis and the expression profile of lncRNA. It may provide new targets to attenuate liver cold ischemia injury through further analyzing the relationship between the apoptosis and lncRNA.

关 键 词：器官保存 冷缺血损伤 长链非编码RNA 细胞凋亡 

分 类 号：R333.4[医药卫生—人体生理学]