急性心肌梗死患者血浆游离线粒体DNA拷贝数定量分析及临床意义  

Quantitative analysis of copy number circulation cell-free mitochondrial DNA in plasma of the patients with acute myocardial infraction and its clinical significance

作　　者：周双艳[1] 杜建明[1] 杜怡清 赵克斌[1] 杨泽华[1] 

机构地区：[1]山西医科大学第一医院医学检验科,太原030001

出　　处：《检验医学与临床》2017年第9期1268-1270,共3页Laboratory Medicine and Clinic

摘　　要：目的探究急性心肌梗死(AMI)后血浆游离线粒体DNA拷贝数变化及其临床意义。方法采集50例健康体检者血浆标本为对照组,50例AMI患者为AMI组,应用荧光定量PCR法测定其循环游离线粒体DNA拷贝数。结果对照组血浆游离线粒体DNA拷贝数为4×10~4(2.5×10~4,9.5×10~4)copies/mL;AMI组血浆游离线粒体DNA拷贝数为2.2×10~5(4.9×10~5,0.8×10~5)copies/mL,差异有统计学意义(P<0.05)。结论 AMI患者血浆游离线粒体DNA拷贝数升高,血浆游离线粒体DNA拷贝数检测可能成为辅助诊断心肌梗死的生物学标志。Objective To explore the changes in the levels of plasma circulation cell-free mitochondrial DNA （mtDNA） in patients with acute myocardial infarction （AMI） and the relationship between myocardial injury markers with its clinical significance.Methods A total of 50 cases of healthy volunteers were selected as control group,and 50 cases with AMI were selected as AMI group.Cell-free mtDNA levels in plasma of the subjects were tested by real-time PCR.Results The expression of circulation cell-free mtDNA in the control group and AMI group were 4×104（2.5×104,9.5×104）copies/mL and 2.2×105（4.9×105,0.8×105）copies/mL,respectively.The difference was statistically significance （P〈0.05）.Conclusion The circulating cell-free mtDNA levels are increased in patients with AMI,circulation cell-free mtDNA levels may serve as a biomarker in the diagnosis of AMI.

关 键 词：急性心肌梗死 线粒体DNA 荧光定量PCR 

分 类 号：R752.53[医药卫生—皮肤病学与性病学]