两种人外周血单核细胞来源树突状细胞表型及功能比较  

Comparison of the phenotypes and function of two different human peripheral blood monocyte-derived dendritic cells

作　　者：张亚娟[1,2] 范璟[1,2] 叶伟[1,2] 王利利[1,2] 殷丹丹[1,2] 蒋文秀[1,2] 赵伟[1,2] ZHANG Ya-juan FAN Jing YE Wei WANG Li-li YING Dan-dan JIANG Wen-xiu ZHAO Wei(The Second Hospital, Southeast University , Nanjing 210003, China Danyang People＇s Hospital, Zhenjiang 212300, China)

机构地区：[1]东南大学附属第二医院,江苏南京210003 [2]丹阳市人民医院,江苏镇江212300

出　　处：《现代医学》2017年第2期169-174,共6页Modern Medical Journal

基　　金：国家自然科学基金资助项目(81402559)

摘　　要：目的:探索体外培养的干扰素-树突状细胞(IFN-DC)和白介素4-树突状细胞(IL-4-DC)在表型及功能上的区别。方法:人外周血单核细胞在体外分别用巨噬细胞集落刺激因子(GM-CSF)联合IFN-α或者GM-CSF联合IL-4诱导培养为IFN-DC或IL-4-DC,随后加入TNF-α促进DCs成熟,流式细胞仪检测两种DC的表型;用负载CMVpp65蛋白的两种DCs刺激自体T淋巴细胞激活,细胞内染色方法检测分泌IFN-γ的T细胞的比例。结果:IFN-DC和IL-4-DC均具有典型的树突状细胞形态和免疫表型,但IFN-DC表达更高水平的CD80、CD83、CD86。负载CMV pp65蛋白的不成熟IFN-DC及不成熟IL-4-DC能够分别诱导(0.28±0.07)%及(0.12±0.02)%的自体CD8+T细胞激活,两者差异有统计学意义(P<0.05);成熟IFN-DC及成熟IL-4-DC能够分别激活(1.03±0.21)%及(0.35±0.14)%的自体CD8+T细胞激活,两者差异有统计学意义(P<0.05)。结论:与IL-4-DC相比,IFN-DC表达更高水平的CD80、CD83、CD86,IFN-DC负载CMV pp65蛋白能够更有效激活人抗原特异性CD8+T细胞。To explore the different phenotypes and function between IFN-DC and IL-4-DC cultured in vitro. Methods The IFN-DC and IL-4-DC were cultured from human peripheral blood monocytes with cytokines GM-CSF plus IFN-α or GM-CSF plus IL-4 in vitro. Then TNF-α were added into the system to induce the maturation. Flow cytometry was used to detect the phenotypes of DCs. Two different DCs loaded with CMVpp65 protein were used to stimulate the proliferation of autologous T lymphocytes. The percentages of T cells secreting IFN-γ were detected by flow cytometry intracellular staining. Results Both of the cultured cells had the typical morphology and immunophenotypes of dendritic cells. However IFN-DC expressed higher levels of CD80, CD83, CD86 compared with IL-4-DC. The percentage of autologous CD8^＋ T cells stimulated by immature IFN-DC loaded with CMVpp65 protein was higher than that by immature IL-4-DC[（0.28±0.07）% vs.（0.12±0.02）%] （P〈0.05）. The effective activation rate of CD8^＋ T cells stumulated by mature IFN-DC was also higher compared with the mature IL-4 DC[（1.03±0.21）% vs.（0.35±0.14）% ] （P〈0.05）. Conclusion IFN-DC could express higher levels of CD80, CD83, CD86 than IL-4-DC. When loaded with CMVpp65 protein IFN-DC could activate human CD8^＋ T cells more effectively.

关 键 词：树突状细胞 体外培养 抗原特异性T细胞 

分 类 号：R392.12[医药卫生—免疫学]