基于聚L-亮氨酸/DNA双层纳米薄膜的对乙酰氨基酚电化学传感器  

Acetaminophen electrochemical sensor based on poly(L-leucine)/DNA nanocomposite bi-layer film

作　　者：杨武[1] 王艳妮[1] 郭昊[1] 郭婧[1] 赵斌[1] 

机构地区：[1]西北师范大学化学化工学院,甘肃兰州730070

出　　处：《西北师范大学学报（自然科学版）》2017年第3期51-57,共7页Journal of Northwest Normal University(Natural Science)

基　　金：国家自然科学基金资助项目(21665024)

摘　　要：利用电沉积法成功制备了新型的聚L-亮氨酸(Pl-LEU)/DNA双层纳米薄膜修饰电极.采用扫描电镜(SEM)和电化学阻抗(EIS)对修饰电极进行了表征,并用循环伏安(CV)和微分脉冲伏安法(DPV)研究了对乙酰氨基酚(AC)在修饰电极上的电化学行为.结果表明,在最优条件下,该修饰电极对AC表现出优越的电催化活性,氧化峰电流与AC浓度在0.7~250μmol·L^(-1)范围内呈现良好的线性关系,检测限为2.3×10^(-9)mol·L^(-1)(S/N=3).此外,该修饰电极还具有较高的灵敏度,较好的稳定性和重现性,可用于实际药物中AC的快速检测.A new type of nanocomposite bi-layer film modified electrode is successfully constructed based onpoly（L-leucine）/DNA using electrodepostion method. The modified electrode is characterized by scanningelectron microscope（SEM） and electrochemical impedance spectroscopy（EIS）. The electrochemicalproperties of acetaminophen（AC） on the modified electrode are studied by cyclic voltammetry（CV） anddifferential pulse vohammetry（DPV）. The results show that the modified electrode displays excellentelectrochemical catalytic activities toward the oxidation of AC under the optimized conditions. Theoxidation current of AC is directly proportional to the concentration of AC in the range of 0.7 to 250 mol·L-1 and the detection limit is 2.3×10^（-9） mol. L 1（S/N=3）. In addition, the modified electrode showshigh sensitivity, stability and reproducibility. Moreover, the modified electrode has been applied for thedetermination of AC in commercial tablets.

关 键 词：聚L-亮氨酸 DNA 电沉积 对乙酰氨基酚(AC) 

分 类 号：O657.14[理学—分析化学]