运动干预对帕金森病大鼠黑质DJ-1的表达和抗氧化物酶的影响  

Effects of exercise intervention on the expresstion of DJ-1 and antioxidant enzymes in the substantia nigra of Parkinson's disease rats

作　　者：王宗兵[1,2] 刘晓莉[1] 乔德才[1] 

机构地区：[1]北京师范大学体育与运动学院,北京100875 [2]西北师范大学体育学院,甘肃兰州730070

出　　处：《西北师范大学学报（自然科学版）》2017年第3期100-105,共6页Journal of Northwest Normal University(Natural Science)

基　　金：国家自然科学基金资助项目(31340025;31571221)

摘　　要：探讨早期运动干预对帕金森病(PD)模型大鼠黑质多巴胺(DA)能神经元的抗氧化保护机制.选清洁级SD大鼠64只分为4组:假手术组(Control,12只)、假手术运动组(Control+Ex,12只)、PD组(PD,20只)和PD运动组(PD+Ex,20只).单侧前脑内侧束(MFB)注射6-OHDA建立PD模型大鼠,运动组术后24h开始进行持续4周跑台训练,11m·min^(-1) ,30min·d^(-1) ,5d·(7d)^(-1) .采用阿扑吗啡(APO)诱导旋转检测行为变化,应用PCR和Western blotting检测黑质DJ-1变化,比色法检测黑质超氧化物歧化酶(SOD)、谷胱甘肽过氧化物酶(GSH-Px)和丙二醛(MDA)指标.黑质DJ-1基因表达显示,PD+Ex组比PD组分别明显增加(P<0.05);黑质DJ-1蛋白表达显示,PD+Ex组较PD组有明显提升(P<0.05);PD组大鼠SOD、GSH-Px活力明显低于Control组(P<0.01),MDA含量显著高于Control组(P<0.01);PD+Ex组大鼠SOD、GSH-Px活力和MDA含量与Control组和PD组均有显著性差异(P<0.05).表明早期运动干预能提高黑质DA能神经元DJ-1并增强抗氧化应激能力.To study the effects of early exersice intervention on oxidative stress of dopaminergic neurons of a rat model of Parkinson＇s disease induced by 6 hydroxydopamine（6 OHDA）. Male Sprague Dawley rats are randomly divided into four groups： Controlgroup（n=12）, Control-flEx group（n=12）, PDgroup（n =20）, PD＋Ex group（n：20）. Parkinson＇s rats are made by injecting 6-hydroxydopamine into medial forebrain bundle（MFB） in the right brain of rat. The trained groups are put on a treadmill to run for 30 min at the speed of 11m＇min ,5 d.（7 d） 1 for four weeks. Injected0.5 mg.kg -1 apomorphine to induce rotation after one week. Four weeks later, observed DJ-1 positive cells lay PCR and western laiotting and chromatometric method tested the activity of superoxide dismutase （SOD） and glutathione peroxidase（GSH-Px） as well as the contents of malondialdehyde（MDA） in substantia nigra. After operation 2 weeks and 4 weeks the number of abnormal rotation is decreased significantly in PDEx group than that in PD group（P〈0.05）. DJ-1 positive cells are significantly decrease in PD group more than those of the control group（P〈0.01） and significantly increase in PD-Ex group than PD group（P〈0.05）. PD group the activities of GSH-Px and SOD are decreased and MDA is increased in substantia nigra with control group （P〈0.01）. Compared to PD group and PD-Ex group, early exersice intervention significantly elevated the activities of GSH-Px and SOD in substantia nigra , while the MDA contents is reduced（P〈0.05）. Early exersice intervention increase the survival rate of DA neurons in substantia nigra of PD model rats, the mechanism is that exercise can improve the substantia nigra DA neurons DJ-1 related to oxidative stress resistance.

关 键 词：帕金森病 黑质 运动干预 DJ-1 氧化应激 

分 类 号：R339.4[医药卫生—人体生理学]