高效液相色谱法同时测定市售醒酒护肝产品中9种天然功效成分的方法建立  

Simultaneous Determination of 9 Natural Ingredientsin Functional Food for Anti-hangover and Hepatoprotection by High Performance Liquid Chromatography

作　　者：邹海民[1,2] 周琛[1] 孙成均[1] 李永新[1] 杨晓松[2] 文君[2] 曾红燕[1] 

机构地区：[1]四川大学华西公共卫生学院卫生检验与检疫系,成都610041 [2]成都市疾病预防控制中心,成都610047

出　　处：《四川大学学报（医学版）》2017年第3期464-470,共7页Journal of Sichuan University(Medical Sciences)

基　　金：科技部"十二.五"科技支撑计划项目(No.2012BAD33B02)资助

摘　　要：目的利用高效液相色谱法同时检测市售醒酒护肝产品中9种天然功效成分(葛根素、槲皮素、水飞蓟宾、五味子醇甲、姜黄素、二氢丹参酮Ⅰ、隐丹参酮、丹参酮Ⅰ和丹参酮ⅡA)。方法样品经体积分数为90%的乙醇超声提取,10 000r/min离心10min,取上清液进样,经C_(18)柱用甲醇-水(用H_3PO_4调pH为2.5)进行梯度洗脱,柱温20℃,流速为0.8mL/min。色谱峰保留时间定性,外标标准曲线法定量。结果在优化的条件下,9种组分在各自的线性范围内相关系数(r)≥0.998,检出限和定量限分别为0.38~0.73mg/kg[信噪比(S/N)=3]和1.27~2.43mg/kg(S/N=10),加标回收率为88.9%~103.2%,加标样品的相对标准偏差为1.3%~3.7%。结论本研究成功建立市售保健品中多类醒酒护肝功效成分的高效液相色谱同时测定方法,能够满足市售醒酒护肝产品的常规分析和质量评价要求。Objective To develop a rapid method of high performance liquid chromatography coupled with variable wave length UV detection for simultaneous determination of 9 natural functional ingredients including puerarin, silymarin, quercetin hydrate, schisandrol A, curcumin, tanshinone I , tanshinoneH A, cryptotanshinone, and dihydrotanshinone I in functional food for anti-hangover and hepatoprotection. Methods The samples were ultrasonically extracted with 90 % ethanol （V/V） and centrifuged at 10 000 r/min for 10 min prior to HPLC analysis. The nine target analytes were separated on a C=8 column with gradient elution using methanol and water （The pH value was adjusted to 2.5 using H3PO4 ） as the mobile phase. Qualitative analysis was carried out using retention times of the chromatographic peaks, while the external standard curves were established for quantification. Results Under the optimal analytical conditions, the peak area of each analyte and its concentration had a good correlation within the linear range （r=0. 998）. The limits of detection and quantification of the method were 0.38- 0.73 mg/kg （S/N=3） and 1.27-2.43 mg/kg （S/N=IO）, respectively. The spiked recoveries of the analytes were 88.9%-103. 2%, and the relative standard deviations were 1. 3-3. 7%. Conclusion The method for the determination of 9 functional ingredients in functional food for anti-hangover and hepatoprotection was proposed for the first time in this study. The results showed that it could meet the requirement of routine analysis and quality control and evaluation.

关 键 词：高效液相色谱法 葛根素 槲皮素 水飞蓟宾 五味子醇甲 姜黄素 丹参酮 

分 类 号：R286.0[医药卫生—中药学]