葛根芩连汤中小檗碱的中药生物药剂学分类系统属性研究  

Biopharmaceutics classification system of Chinese materia medica of berberine in Gegen Qinlian decoction

作　　者：刘洋[1] 朱美玲[1] 孙慧娟[1] 王子禹 陈江鹏[1] 董月柳 董玲[1] 

机构地区：[1]北京中医药大学,北京100102

出　　处：《中国中药杂志》2017年第8期1545-1550,共6页China Journal of Chinese Materia Medica

基　　金：国家自然科学基金项目(81473362)

摘　　要：中药生物药剂学分类系统(CMMBCS)的顶层研究之一是中药复方对单一成分的影响研究,除单成分自身溶解性和渗透性外,需结合复方中多成分环境下其溶解性和渗透性各自提升度来进行分类。该文主要是研究葛根芩连复方中小檗碱的CMMBCS分类属性的变化,即小檗碱在葛根芩连汤中的溶解性和渗透性,考察其在整个复方环境中的变化及分类。采用经典摇瓶法和高效液相色谱法测定其在复方中的溶解度变化,并采用大鼠离体外翻肠囊实验进行小檗碱肠吸收定性,采用在体单向灌流法进行小檗碱肠吸收定量研究,同时和肠道血管并行采血实验进行吸收入血的定性和定量研究。One of the top-level researches of biopharmaceutics classification system of Chinese materia medica （CMMBCS） is the study on single component in compound Chinese medicine.The medicines shall be classified according to its solubility and intestinal permeability, as well as the ascending degree in multicomponent environment.Based on above, we chose berberine as the main object to explore the change rules of its solubility and intestinal permeability in Gegen Qinlian decoction.Shaking flask-HPLC was used to detect the solubility changes of berberine in compounds.The qualitative investigation of berberine in intestinal absorption was measured by everted gut sac, and the quantitative research of berberine in intestinal absorption was measured by single-pass intestinal perfusion experiment, while the qualitative and quantitative research of berberine absorption into blood was measured by in intestinal perfusion with venous sampling experiment.

关 键 词：葛根芩连汤 小檗碱 溶解性 肠渗透性 中药生物药剂学分类系统 

分 类 号：R283.6[医药卫生—中药学]