G-CSF对豚鼠单个缺血心房肌细胞钙、钠、钾电流的影响  

Effects of G-CSF on calcium,sodium and potassium ion channel currents of ischemic atrial myocytes in guinea pig

作　　者：罗涛[1] 郭梅[2] 史成龙[1] 周敏[1] 贾忠伟[1] 浦奎[1] 

机构地区：[1]中国人民解放军第254医院心血管内科,天津300142 [2]中国人民解放军第254医院检验科,天津300142

出　　处：《中国病理生理杂志》2017年第5期857-864,共8页Chinese Journal of Pathophysiology

摘　　要：目的:采用膜片钳全细胞记录方法,观察粒细胞集落刺激因子(G-CSF)急性干预对分离的豚鼠单个缺血心房肌细胞钙、钠、钾电流的影响。方法:使用酶解方法获得豚鼠单个心房肌细胞,在缺血缺氧条件下,采用全细胞膜片钳技术观察记录不同浓度G-CSF急性干预对钙通道电流-电压曲线、激活曲线、失活曲线,钠通道电流-电压曲线、激活曲线、失活曲线、静态失活曲线,延迟整流钾通道及其快成分、慢成分电流-电压曲线的影响。结果:随G-CSF浓度的增加,缺血心房肌细胞膜L型钙通道电流较缺血对照组有明显增大。当G-CSF浓度超过100μg/kg后,L型钙通道电流的增强维持于同一水平。当给予G-CSF浓度为300μg/kg时最大激活电压发生改变,较前有所增加。激活曲线与失活曲线未见明显改变。不同浓度G-CSF对缺血心房肌细胞膜钠离子通道电流无明显影响。G-CSF干预缺血心房肌细胞膜延迟整流钾电流未见明显改变,但延迟整流钾电流快成分在G-CSF 100μg/kg干预后明显增加,而延迟整流钾电流慢成分未见明显改变。结论:G-CSF对于缺血心房肌细胞部分通道的作用影响基本为非电压依赖性,但具有浓度依赖性,可能减少缺血心房心律失常的发生率。AIM： To observe the effects of granulocyte colony-stimulating factor （G-CSF） on calcium, sodium and potassium ion channel currents of the ischemic atrial myocytes in guinea pig by whole-cell patch clamp technique.METHODS： The guinea pig atrial myocytes were obtained by enzymolysis. Under ischemia and hypoxia condition, whole-cell patch clamp was used to observe the effects of G-CSF at various concentrations on the changes of the I-V curve, activation curve and availability of L-type calcium channel current （ICa,L） and voltage-dependent sodium channel current （INa）, as well as I-V curve of delayed rectifier potassium channel current （IK）.RESULTS： Under ischemic condition, the I-V curves of ICa,L were changed by acute G-CSF intervention in a dose-dependent fashion. Except for G-CSF at dose of 300 μg/kg, the other concentrations of G-CSF did not change the activation curve and availability of ICa,L, indicating that the effects of G-CSF on ICa,L were in a voltage-independent fashion. The I-V curves of ICa,L under ischemic condition were gradually approaching the normal levels by the higher dose of G-CSF, while the effect of 300 μg/kg G-CSF on ICa,L was similar to 100 μg/kg G-CSF. Acute G-CSF intervention at different doses did not change I-V curve, activation curve, and availability or steady-state availability of INa. As a part of IK, the rapid activating component （IKr） was improved by 100 μg/kg and 300 μg/kg G-CSF intervention with the similar effects, while the slowly activating component （IKs） was not changed by G-CSF.CONCLUSION： G-CSF affects ion channel electrophysiological properties of ischemic atrial myocytes in a voltage-independent but concentration-dependent manner, thus reducing the incidence of atrial arrhythmia.

关 键 词：粒细胞集落刺激因子 钠通道 L型钙通道 延迟整流钾通道 心房肌细胞 

分 类 号：R541.7[医药卫生—心血管疾病] R329.24[医药卫生—内科学]