白藜芦醇对花萼海绵诱癌素所致小鼠空间记忆障碍的预防作用  

Prevention of resveratrol on spatial memory loss of mice induced by calyculin A

作　　者：李颖[1] 向瑶[1] 邓惠萍[1] 朱婷婷[1] 赵玉蓉[1] 李夏春[1] 

机构地区：[1]三峡大学医学院生理学与病理生理学系,湖北宜昌443002

出　　处：《中国病理生理杂志》2017年第5期951-955,共5页Chinese Journal of Pathophysiology

基　　金：三峡大学博士启动基金(No.KJ2013B056)

摘　　要：目的:探讨腹腔注射白藜芦醇对昆明小鼠侧脑室注射花萼海绵诱癌素引起的空间记忆障是否有预防作用。方法:2月龄昆明小鼠44只随机分为4组,分别为生理盐水对照组、模型组、白藜芦醇低剂量组和白藜芦醇高剂量组;生理盐水对照组和模型组腹腔注射等体积生理盐水,白藜芦醇低剂量组和白藜芦醇高剂量组分别腹腔注射白藜芦醇5 mg/kg和10 mg/kg共21 d,除对照组外,第22天从侧脑室注射花萼海绵诱癌素4μL,第27天进行Morris水迷宫定位航行训练,持续6 d,24 h后进行寻找平台潜伏期检测。再处死小鼠,进行灌流固定做高尔基染色观察神经元树突和树突棘,并取新鲜脑海马匀浆后检测SOD活性。结果:白藜芦醇低剂量组显著缩短侧脑室注射花萼海绵诱癌素引起的逃避潜伏期。白藜芦醇低剂量组显著增加侧脑室注射花萼海绵诱癌素引起的CA1区锥体神经元树突的长度和分枝数目。白藜芦醇低剂量组显著增加侧脑室注射花萼海绵诱癌素引起的海马CA1区锥体神经元树突棘的密度。白藜芦醇高剂量组显著增加花萼海绵诱癌素引起的海马SOD活性,而低剂量组并不改变SOD活性。结论:低剂量白藜芦醇对侧脑室注射花萼海绵诱癌素引起的空间记忆障碍具有预防作用,其机制可能与减轻花萼海绵诱癌素引起的树突损伤有关。AIM： To explore the preventive effect of resveratrol on spatial memory loss of the mice induced by intralateroventricular injection of calyculin A （CA）.METHODS： Kunming mice of 2 months （n=44） were divided into saline control group, CA group, low-dose resveratrol group and high-dose resveratrol group. The mice in control group and CA group were intraperitoneally injected with equal volume of saline for 21 d, while the mice in low-dose resveratrol group and high-dose resveratrol group were intraperitoneally injected with resveratrol at 5 mg/kg and 10 mg/kg, respectively. At 22 d, CA （4 μL） was injected into the lateral ventricles in CA group, low-dose resveratrol group and high-dose resveratrol group. Morris water maze test was applied to examine the changes of learning and memory abilities of the mice at 27 d. The Golgi staining was used to observe the morphological changes of dendrites and dendritic spines. The hippocampal tissues were homogenated to detect SOD activity.RESULTS： Low-dose resveratrol significantly decreased the escape latency delay induced by CA. Low-dose resveratrol attenuated the decreases in the number of dendrites and the density of dendritic spines of neurons in hippocampal CA1 region induced by CA. High-dose resveratrol but not low-dose resveratrol attenuated the decreased SOD activity induced by CA.CONCLUSION： Resveratrol at low dose attenuates memory loss in the mice induced by CA though preventing dendrite injury.

关 键 词：白藜芦醇 花萼海绵诱癌素 空间记忆 树突 

分 类 号：R965[医药卫生—药理学] R749.29[医药卫生—药学]